Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis.

IF 5.7 2区医学 Q1 Medicine

Clinical Epigenetics Pub Date : 2023-06-27 DOI:10.1186/s13148-023-01521-w

Ledio Bocova, Wouter Hubens, Cordula Engel, Steffen Koschmieder, Edgar Jost, Wolfgang Wagner

引用次数: 1

Abstract

Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34⁺ cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia.

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用靶向DNA甲基化分析定量造血干细胞和祖细胞。

造血干细胞和祖细胞(HSPCs)在日常临床实践中被流式细胞术量化。在这项研究中，我们提供了概念证明，HSPCs也可以通过靶向DNA甲基化(DNAm)分析来估计。MYO1D、STK17A和SP140基因中三个单独的CG二核苷酸(CpG位点)上的dna水平与动员外周血中CD34+细胞数量和白血病细胞计数相关。在未来，这种表观遗传生物标志物可以支持白血病干细胞动员、HSPC收获或原细胞计数的评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Epigenetics Biochemistry, Genetics and Molecular Biology-Developmental Biology

CiteScore

8.90

自引率

5.30%

发文量

150

审稿时长

12 weeks

期刊介绍： Clinical Epigenetics, the official journal of the Clinical Epigenetics Society, is an open access, peer-reviewed journal that encompasses all aspects of epigenetic principles and mechanisms in relation to human disease, diagnosis and therapy. Clinical trials and research in disease model organisms are particularly welcome.