Comparative Evaluation of Fibrin Network Formation after Root Conditioning using Erbium, Chromium-Doped Yttrium Scandium Gallium and Garnet Laser, Ethylene-Diamine-Tetra-Acetic Acid, and Tetracycline on Dentin: A Scanning Electron Microscopic Study.

IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Contemporary Clinical Dentistry Pub Date : 2023-01-01 Epub Date: 2023-02-07 DOI:10.4103/ccd.ccd_626_21

Riya Liza Satish, Maya Rajan Peter, Anuradha Bhaskar, Rajesh Vylopillil, Biju Balakrishnan, Reshma Suresh

{"title":"Comparative Evaluation of Fibrin Network Formation after Root Conditioning using Erbium, Chromium-Doped Yttrium Scandium Gallium and Garnet Laser, Ethylene-Diamine-Tetra-Acetic Acid, and Tetracycline on Dentin: A Scanning Electron Microscopic Study.","authors":"Riya Liza Satish, Maya Rajan Peter, Anuradha Bhaskar, Rajesh Vylopillil, Biju Balakrishnan, Reshma Suresh","doi":"10.4103/ccd.ccd_626_21","DOIUrl":null,"url":null,"abstract":"Context: Regeneration of periodontal tissues depend on the adhesion and development of fibrin clots to the root surface. Demineralization of the root surface ensures removal of smear layer uncovering dentin tubules and collagen matrix. Root conditioning agents were introduced to remove the smear layer and enhance the adhesion of blood components.Aims: The aim of the study was to determine the effect of erbium, chromium-doped yttrium, scandium, gallium and garnet (Er, Cr: YSGG) laser on smear layer removal (SLR) and fibrin network formation when compared to tetracycline, and ethylene-diamine-tetra-acetic acid (EDTA) for periodontal regeneration.Settings and design: The study was conducted at Amrita school of dentistry and the study design involves in vitro comparative study.Subjects and methods: Forty dentinal sections were prepared from freshly extracted teeth that were periodontally affected. The samples were divided into four groups: Scaling and root planing (SRP), tetracycline, EDTA, and Er, Cr: YSGG laser. The samples were assessed using a scanning electron microscope and photomicrographs were taken and analyzed for removal of smear layer, blood component adhesion (BCA), and fibrin network formation.Statistical analysis used: Analysis of variance test and Kruskal-Wallis test with P < 0.05 considered to be statistically significant.Results: There was a significant difference between Er, Cr: YSGG laser, tetracycline hydrochloride, and EDTA categories when compared with SRP categories. There was no significant difference between Er, Cr: YSGG laser, tetracycline, and EDTA according to SLR scoring and BCA scoring.Conclusions: It can be concluded that Er, Cr: YSGG laser-treated samples presented complete elimination of smear layer, formation of fibrin network, and BCA along with desensitizing effect for a better reduction in dentin hypersensitivity.","PeriodicalId":10632,"journal":{"name":"Contemporary Clinical Dentistry","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e3/f1/CCD-14-72.PMC10209766.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Contemporary Clinical Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ccd.ccd_626_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/2/7 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Context: Regeneration of periodontal tissues depend on the adhesion and development of fibrin clots to the root surface. Demineralization of the root surface ensures removal of smear layer uncovering dentin tubules and collagen matrix. Root conditioning agents were introduced to remove the smear layer and enhance the adhesion of blood components.

Aims: The aim of the study was to determine the effect of erbium, chromium-doped yttrium, scandium, gallium and garnet (Er, Cr: YSGG) laser on smear layer removal (SLR) and fibrin network formation when compared to tetracycline, and ethylene-diamine-tetra-acetic acid (EDTA) for periodontal regeneration.

Settings and design: The study was conducted at Amrita school of dentistry and the study design involves in vitro comparative study.

Subjects and methods: Forty dentinal sections were prepared from freshly extracted teeth that were periodontally affected. The samples were divided into four groups: Scaling and root planing (SRP), tetracycline, EDTA, and Er, Cr: YSGG laser. The samples were assessed using a scanning electron microscope and photomicrographs were taken and analyzed for removal of smear layer, blood component adhesion (BCA), and fibrin network formation.

Statistical analysis used: Analysis of variance test and Kruskal-Wallis test with P < 0.05 considered to be statistically significant.

Results: There was a significant difference between Er, Cr: YSGG laser, tetracycline hydrochloride, and EDTA categories when compared with SRP categories. There was no significant difference between Er, Cr: YSGG laser, tetracycline, and EDTA according to SLR scoring and BCA scoring.

Conclusions: It can be concluded that Er, Cr: YSGG laser-treated samples presented complete elimination of smear layer, formation of fibrin network, and BCA along with desensitizing effect for a better reduction in dentin hypersensitivity.

Abstract Image

查看原文本刊更多论文

使用Er、铬掺杂的钇、钪和石榴石激光、乙二胺四乙酸和四环素对牙本质进行根处理后纤维蛋白网络形成的比较评估：扫描电子显微镜研究。

背景：牙周组织的再生取决于纤维蛋白凝块对根表面的粘附和发展。根表面的去矿化可确保去除覆盖牙本质小管和胶原基质的涂抹层。根调节剂被引入以去除涂抹层并增强血液成分的粘附。目的：与四环素和乙二胺四乙酸（EDTA）用于牙周再生相比，本研究的目的是确定掺铒、铬钇、钪、镓和石榴石（Er，Cr:YSGG）激光对涂抹层去除（SLR）和纤维蛋白网络形成的影响。设置和设计：该研究在Amrita牙科学院进行，研究设计涉及体外比较研究。受试者和方法：从刚拔出的受牙周影响的牙齿中制备40个牙本质切片。将样品分为四组：刮根刨根（SRP）、四环素、EDTA和Er，Cr:YSGG激光。使用扫描电子显微镜评估样品，并拍摄显微照片并分析涂抹层的去除、血液成分粘附（BCA）和纤维蛋白网络的形成。使用的统计分析：方差分析和Kruskal-Wallis检验，P<0.05被认为具有统计学意义。结果：Er、Cr:YSGG激光、盐酸四环素和EDTA类别与SRP类别相比有显著差异。根据SLR评分和BCA评分，Er、Cr:YSGG激光、四环素和EDTA之间没有显著差异。结论：Er、Cr:YSGG激光治疗的牙本质标本具有完全消除涂抹层、形成纤维蛋白网络和BCA的作用，并具有脱敏作用，可更好地降低牙本质超敏反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Contemporary Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： The journal Contemporary Clinical Dentistry (CCD) (Print ISSN: 0976-237X, E-ISSN:0976- 2361) is peer-reviewed journal published on behalf of Maharishi Markandeshwar University and issues are published quarterly in the last week of March, June, September and December. The Journal publishes Original research papers, clinical studies, case series strictly of clinical interest. Manuscripts are invited from all specialties of Dentistry i.e. Conservative dentistry and Endodontics, Dentofacial orthopedics and Orthodontics, Oral medicine and Radiology, Oral pathology, Oral surgery, Orodental diseases, Pediatric Dentistry, Periodontics, Clinical aspects of Public Health dentistry and Prosthodontics. Review articles are not accepted. Review, if published, will only be by invitation from eminent scholars and academicians of National and International repute in the field of Medical/Dental education.