Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitis.

IF 11.3 1区 医学 Q1 Medicine
Ying Zhang, Hengyu Lei, Pengchong Wang, Qinyuan Zhou, Jie Yu, Xue Leng, Ruirui Ma, Danyang Wang, Kai Dong, Jianfeng Xing, Yalin Dong
{"title":"Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitis.","authors":"Ying Zhang,&nbsp;Hengyu Lei,&nbsp;Pengchong Wang,&nbsp;Qinyuan Zhou,&nbsp;Jie Yu,&nbsp;Xue Leng,&nbsp;Ruirui Ma,&nbsp;Danyang Wang,&nbsp;Kai Dong,&nbsp;Jianfeng Xing,&nbsp;Yalin Dong","doi":"10.1186/s40824-023-00412-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Reactive oxygen species (ROS) overproduction and excessive hypoxia play pivotal roles in the initiation and progression of ulcerative colitis (UC). Synergistic ROS scavenging and generating O<sub>2</sub> could be a promising strategy for UC treatment.</p><p><strong>Methods: </strong>Ceria nanozymes (PEG-CNPs) are fabricated using a modified reverse micelle method. We investigate hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells and RAW 264.7 macrophages and their effects on pro-inflammatory macrophages activation. Subsequently, we investigate the biodistribution, pharmacokinetic properties and long-term toxicity of PEG-CNPs in mice. PEG-CNPs are administered intravenously to mice with 2,4,6-trinitrobenzenesulfonic acid-induced colitis to test their colonic tissue targeting and assess their anti-inflammatory activity and mucosal healing properties in UC.</p><p><strong>Results: </strong>PEG-CNPs exhibit multi-enzymatic activity that can scavenge ROS and generate O<sub>2</sub>, promote intestinal epithelial cell healing and inhibit pro-inflammatory macrophage activation, and have good biocompatibility. After intravenous administration of PEG-CNPs to colitis mice, they can enrich at the site of colonic inflammation, and reduce hypoxia-induced factor-1α expression in intestinal epithelial cells by scavenging ROS to generate O<sub>2</sub>, thus further promoting disrupted intestinal mucosal barrier restoration. Meanwhile, PEG-CNPs can effectively scavenge ROS in impaired colon tissues and relieve colonic macrophage hypoxia to suppress the pro-inflammatory macrophages activation, thereby preventing UC occurrence and development.</p><p><strong>Conclusion: </strong>This study has provided a paradigm to utilize metallic nanozymes, and suggests that further materials engineering investigations could yield a facile method based on the pathological characteristics of UC for clinically managing UC.</p>","PeriodicalId":9079,"journal":{"name":"Biomaterials Research","volume":null,"pages":null},"PeriodicalIF":11.3000,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375752/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s40824-023-00412-8","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 1

Abstract

Background: Reactive oxygen species (ROS) overproduction and excessive hypoxia play pivotal roles in the initiation and progression of ulcerative colitis (UC). Synergistic ROS scavenging and generating O2 could be a promising strategy for UC treatment.

Methods: Ceria nanozymes (PEG-CNPs) are fabricated using a modified reverse micelle method. We investigate hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells and RAW 264.7 macrophages and their effects on pro-inflammatory macrophages activation. Subsequently, we investigate the biodistribution, pharmacokinetic properties and long-term toxicity of PEG-CNPs in mice. PEG-CNPs are administered intravenously to mice with 2,4,6-trinitrobenzenesulfonic acid-induced colitis to test their colonic tissue targeting and assess their anti-inflammatory activity and mucosal healing properties in UC.

Results: PEG-CNPs exhibit multi-enzymatic activity that can scavenge ROS and generate O2, promote intestinal epithelial cell healing and inhibit pro-inflammatory macrophage activation, and have good biocompatibility. After intravenous administration of PEG-CNPs to colitis mice, they can enrich at the site of colonic inflammation, and reduce hypoxia-induced factor-1α expression in intestinal epithelial cells by scavenging ROS to generate O2, thus further promoting disrupted intestinal mucosal barrier restoration. Meanwhile, PEG-CNPs can effectively scavenge ROS in impaired colon tissues and relieve colonic macrophage hypoxia to suppress the pro-inflammatory macrophages activation, thereby preventing UC occurrence and development.

Conclusion: This study has provided a paradigm to utilize metallic nanozymes, and suggests that further materials engineering investigations could yield a facile method based on the pathological characteristics of UC for clinically managing UC.

Abstract Image

Abstract Image

Abstract Image

通过协同改善溃疡性结肠炎患者的缺氧和清除活性氧,恢复失调的肠屏障和炎症调节。
背景:活性氧(ROS)的过度产生和过度缺氧在溃疡性结肠炎(UC)的发生和发展中起着关键作用。协同ROS清除和生成O2可能是UC治疗的有前途的策略。方法:采用改进的反胶束法制备二氧化铈纳米酶(PEG-CNPs)。我们研究了肠上皮细胞和RAW 264.7巨噬细胞中PEG-CNPs的缺氧减弱和ROS清除及其对促炎巨噬细胞激活的影响。随后,我们研究了PEG-CNPs在小鼠体内的生物分布、药代动力学特性和长期毒性。将PEG-CNPs静脉给予2,4,6-三硝基苯磺酸诱导的结肠炎小鼠,以测试其结肠组织靶向性,并评估其在UC中的抗炎活性和粘膜愈合特性。结果:PEG-CNPs具有清除ROS、生成O2、促进肠上皮细胞愈合、抑制促炎巨噬细胞活化的多酶活性,具有良好的生物相容性。结肠炎小鼠经静脉给药后,PEG-CNPs可在结肠炎症部位富集,通过清除ROS生成O2,降低缺氧诱导的肠上皮细胞中因子-1α的表达,从而进一步促进被破坏的肠粘膜屏障的恢复。同时,PEG-CNPs可以有效清除受损结肠组织中的ROS,缓解结肠巨噬细胞缺氧,抑制促炎巨噬细胞活化,从而防止UC的发生发展。结论:本研究为利用金属纳米酶提供了一个范例,并表明进一步的材料工程研究可以根据UC的病理特征产生一种简便的方法来临床治疗UC。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信