The chromatin - triple helix connection.

IF 2.9 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biological Chemistry Pub Date : 2023-07-31 Print Date: 2023-10-26 DOI:10.1515/hsz-2023-0189
Rodrigo Maldonado, Gernot Längst
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引用次数: 0

Abstract

Mammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shaping higher-order genome organisation, such as involvement in long-range enhancer-promoter interactions, transcription hubs, heterochromatin, nuclear bodies and phase transitions. Different mechanisms allow the tethering of these chromatin-associated RNAs (caRNA) to chromosomes, including RNA binding proteins, the RNA polymerases and R-loops. In this review, we focus on the sequence-specific targeting of RNA to DNA by forming triple helical structures and describe its interplay with chromatin. It turns out that nucleosome positioning at triple helix target sites and the nucleosome itself are essential factors in determining the formation and stability of triple helices. The histone H3-tail plays a critical role in triple helix stabilisation, and the role of its epigenetic modifications in this process is discussed.

染色质-三螺旋连接。
哺乳动物基因组被广泛转录,产生大量编码和非编码转录物。很大一部分核RNA与染色质物理相关,在基因激活和沉默中发挥作用,形成高阶基因组组织,如参与长程增强子-启动子相互作用、转录枢纽、异染色质、核小体和相变。不同的机制允许这些染色质相关RNA(caRNA)与染色体相连,包括RNA结合蛋白、RNA聚合酶和R-环。在这篇综述中,我们重点关注RNA通过形成三螺旋结构对DNA的序列特异性靶向,并描述其与染色质的相互作用。结果表明,核小体在三螺旋靶位点的定位和核小体本身是决定三螺旋形成和稳定性的重要因素。组蛋白H3尾部在三螺旋稳定中起着关键作用,并讨论了其表观遗传学修饰在这一过程中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Chemistry
Biological Chemistry 生物-生化与分子生物学
CiteScore
7.20
自引率
0.00%
发文量
63
审稿时长
4-8 weeks
期刊介绍: Biological Chemistry keeps you up-to-date with all new developments in the molecular life sciences. In addition to original research reports, authoritative reviews written by leading researchers in the field keep you informed about the latest advances in the molecular life sciences. Rapid, yet rigorous reviewing ensures fast access to recent research results of exceptional significance in the biological sciences. Papers are published in a "Just Accepted" format within approx.72 hours of acceptance.
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