The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates

IF 11.5 2区生物学 Q1 GENETICS & HEREDITY

Genomics, Proteomics & Bioinformatics Pub Date : 2023-02-01 DOI:10.1016/j.gpb.2022.12.005

Gang Chen , Salma Mostafa , Zhaogeng Lu , Ran Du , Jiawen Cui , Yun Wang , Qinggang Liao , Jinkai Lu , Xinyu Mao , Bang Chang , Quan Gan , Li Wang , Zhichao Jia , Xiulian Yang , Yingfang Zhu , Jianbin Yan , Biao Jin

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引用次数: 5

Abstract

Jasminum sambac (jasmine flower), a world-renowned plant appreciated for its exceptional flower fragrance, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first de novo genome assembly of J. sambac with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in jasmonate biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of JsTPS3 in β-ocimene biosynthesis, as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for J. sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in Jasminum.

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茉莉花（Jasminum sambac）基因组深入了解花香和茉莉酸盐的生物合成

茉莉花是世界著名的植物，以其独特的花香而闻名，具有重要的文化和经济意义。然而，其香味的遗传基础在很大程度上是未知的。在这里，我们提出了桑巴克的第一个从头基因组组装，550.12Mb（支架N50=40.10Mb）组装成13个假染色体。与花香相关的萜烯合成酶（TPS）基因通过基因组中的串联重复以基因簇的形式被显著扩增。水杨酸/苯甲酸/可可碱（SABATH）和苄醇O-乙酰转移酶/花青素O-羟基肉桂酰转移酶/邻氨基苯甲酸酯N-羟基肉桂酰/苯甲酰转移酶-脱乙酰吲哚啉-4-乙酰转移酶（BAHD）超家族中的基因簇被鉴定与苯丙素/类苯化合物的生物合成有关。参与茉莉酸生物合成的几个关键基因被复制，导致拷贝数增加。此外，多组学分析确定了各种芳香化合物和许多参与香料生物合成途径的基因。此外，JsTPS3在β-ocimene生物合成中的作用，以及JsAOC1和JsAOS在茉莉酸生物合成中的功能得到了验证。本研究中组装的茉莉花基因组为研究茉莉花的花香和茉莉酸生物合成提供了基本的遗传资源，并为茉莉花的功能基因组研究和品种改良奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics, Proteomics & Bioinformatics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

14.30

自引率

4.20%

发文量

844

审稿时长

61 days

期刊介绍： Genomics, Proteomics and Bioinformatics (GPB) is the official journal of the Beijing Institute of Genomics, Chinese Academy of Sciences / China National Center for Bioinformation and Genetics Society of China. It aims to disseminate new developments in the field of omics and bioinformatics, publish high-quality discoveries quickly, and promote open access and online publication. GPB welcomes submissions in all areas of life science, biology, and biomedicine, with a focus on large data acquisition, analysis, and curation. Manuscripts covering omics and related bioinformatics topics are particularly encouraged. GPB is indexed/abstracted by PubMed/MEDLINE, PubMed Central, Scopus, BIOSIS Previews, Chemical Abstracts, CSCD, among others.