Efficacy of Baishao Luoshi decoction on synaptic plasticity in rats with post stroke spasticity.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan Pub Date : 2023-04-01 DOI:10.19852/j.cnki.jtcm.20221108.002

Xie Le, Mao Guo, Xie Yao, Cao Sijia, Zhou Shen, Jiang Junlin, Yao Ting, Fan Jianhu, Liu Dong, Kang Fuliang, W U Dahua, G E Jinwen

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引用次数: 1

Abstract

Objective: To evaluate the efficacy of Baishao Luoshi decoction (, BD) on synaptic plasticity in rats with post stroke spasticity (PSS), and to study the mechanism behind the action.

Methods: The PSS model of rat was established by middle cerebral artery occlusion (MCAO). The neurological deficit symptoms were evaluated by modified neurological deficit score (mNSS). Muscle tension were evaluated by Modified Ashworth score (MAS). Transmission electron microscopy (TEM) was used to observe the synaptic ultrastructure. The expression of synaptic plasticity-related protein brain derived neurotrophic factor (BDNF), growth associated protein-43 (GAP43), synaptophysin (p38) and microtubule-associated protein 2 (MAP2) in the brain tissue around the infarct were detected by Western blotting.

Results: We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD. The thickness of postsynaptic density and the synaptic curvature increased significantly. The expression of synaptic plasticity-related protein BDNF, GAP43, p38, MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.

Conclusions: Alleviating PSS by BD may be related to rescuing the synaptic plasticity, which provides a probable new therapeutic method for PSS.

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白芍螺石汤对脑卒中后痉挛大鼠突触可塑性的影响。

目的:观察白芍螺石汤对脑卒中后痉挛大鼠突触可塑性的影响，并探讨其作用机制。方法:采用大脑中动脉闭塞法(MCAO)建立大鼠PSS模型。采用改良神经功能缺损评分(mNSS)评价神经功能缺损症状。采用改良Ashworth评分(MAS)评价肌肉张力。透射电镜观察突触的超微结构。Western blotting检测梗死周围脑组织突触可塑性相关蛋白脑源性神经营养因子(BDNF)、生长相关蛋白-43 (GAP43)、synaptophysin (p38)和微管相关蛋白2 (MAP2)的表达。结果:我们发现双酚d治疗可显著改善mNSS，改善肢体痉挛，突触后密度厚度和突触曲率明显增加。脑梗死后，脑梗死周围脑组织突触可塑性相关蛋白BDNF、GAP43、p38、MAP2的表达明显升高。结论:脑梗死后减轻PSS可能与恢复突触可塑性有关，为PSS的治疗提供了一种可能的新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan

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