High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Fang-Cheng Jiang, Gao-Qiang Zhai, Jia-Lin Liu, Rui-Gong Wang, Yuan-Ping Yang, Harivignesh Murugesan, Xiao-Xiang Yu, Xiu-Fang Du, Juan He, Zhen-Bo Feng, Shang Ling Pan, Gang Chen, Sheng-Hua Li, Zhi-Guang Huang
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Abstract

The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA-seq) data and in-house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single-cell RNA-seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease-free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis.

Abstract Image

着丝粒蛋白A在前列腺癌症中的高表达及其分子机制和临床意义:基于数据挖掘和免疫组织化学的研究。
癌症(PCa)的进展导致预后不良。然而,PCa的分子机制尚不完全清楚。本研究旨在阐明着丝粒蛋白A(CENPA)在前列腺癌中的重要作用。大量的体RNA测序(RNA-seq)数据和内部免疫组织化学数据用于分析CENPA在前列腺癌和转移性前列腺癌(MPCa)中的表达水平。单细胞RNA-seq数据用于探索CENPA在不同前列腺亚群中的表达状态。采用富集分析法检测CENPA在前列腺癌中的作用。临床病理参数分析用于分析CENPA的临床价值。结果显示,CENPA在PCa(标准化平均差[SMD]=0.83,p=0.001)和MPCa(SMD=0.61,p=0.029)中上调。与AR阳性的上皮细胞相比,CENPA在雄激素受体(AR)阴性的前列腺癌症干细胞(CSCs)中过表达。CENPA可能通过影响细胞周期来影响前列腺癌的发展。淋巴结转移患者CENPA的表达水平较高。CENPA高表达的患者无病生存率较差。总之,CENPA的过表达可能通过调节细胞周期和促进转移来影响前列腺癌的发展。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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