De novo genome assembly of Akanthomyces muscarius, a biocontrol agent of insect agricultural pests.

Access Microbiology Pub Date : 2023-06-12 eCollection Date: 2023-01-01 DOI:10.1099/acmi.0.000568.v3
Zoltan Erdos, David John Studholme, Ben Raymond, Manmohan D Sharma
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Abstract

The entomopathogenic fungus Akanthomyces muscarius is commonly used in agriculture to manage insect pests. Besides its use as a commercially important biological control agent, it also presents a potential model for studying host-pathogen interactions and the evolution of virulence in a laboratory setting. Here, we describe the first high-quality genome sequence for A. muscarius. We used long- and short-read sequencing to assemble a sequence of 36.1 Mb with an N50 of 4.9 Mb. Genome annotation predicted 12347 genes, with 96.6 % completeness based on the core Hypocrealen gene set. The high-quality assembly and annotation of A. muscarius presented in this study provides an essential tool for future research on this commercially important species.

农业害虫生物控制剂 Akanthomyces muscarius 的全新基因组组装。
昆虫病原真菌Akanthomyces muscarius通常用于农业防治害虫。除了作为一种具有重要商业价值的生物防治剂之外,它还是在实验室环境中研究宿主-病原体相互作用和毒力进化的潜在模型。在这里,我们描述了 A. muscarius 的首个高质量基因组序列。我们使用长短线程测序技术组装了一个 36.1 Mb 的序列,N50 为 4.9 Mb。基因组注释预测了 12347 个基因,基于核心 Hypocrealen 基因组的完整率为 96.6%。本研究提供的 A. muscarius 的高质量组装和注释为今后研究这一具有重要商业价值的物种提供了重要工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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