Naringenin attenuates cerebral ischemia/reperfusion injury by inhibiting oxidative stress and inflammatory response via the activation of SIRT1/FOXO1 signaling pathway in vitro.

IF 1.1 4区 医学 Q3 SURGERY
Peng Zhao, Yi Lu, Zhiyun Wang
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Abstract

Purpose: To explore the protection of naringenin against oxygen-glucose deprivation/reperfusion (OGD/R)-induced HT22 cell injury, a cell model of cerebral ischemia/reperfusion (I/R) injury in vitro, focusing on SIRT1/FOXO1 signaling pathway.

Methods: Cytotoxicity, apoptosis, reactive oxygen species (ROS) generation, malondialdehyde (MDA) content, 4-hydroxynonenoic acid (4-HNE) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were measured by commercial kits. Inflammatory cytokines levels were determined by enzyme-linked immunosorbent assay (ELISA). The protein expressions were monitored by Western blot analysis.

Results: Naringenin significantly ameliorated OGD/R-induced cytotoxicity and apoptosis in HT22 cells. Meanwhile, naringenin promoted SIRT1 and FOXO1 protein expressions in OGD/R-subjected HT22 cells. In addition, naringenin attenuated OGD/R-induced cytotoxicity, apoptosis, oxidative stress (the increased ROS, MDA and 4-HNE levels, and the decreased SOD, GSH-Px and CAT activities) and inflammatory response (the increased tumor necrosis factor-α, interleukin [IL]-1β, and IL-6 levels and the decreased IL-10 level), which were blocked by the inhibition of the SIRT1/FOXO1 signaling pathway induced by SIRT1-siRNA transfection.

Conclusions: Naringenin protected HT22 cells against OGD/R injury depending on its antioxidant and anti-inflammatory activities via promoting the SIRT1/FOXO1 signaling pathway.

Abstract Image

Abstract Image

Abstract Image

柚皮素在体外通过激活SIRT1/FOXO1信号通路抑制氧化应激和炎症反应,减轻脑缺血再灌注损伤。
目的:探讨柚皮素对体外脑缺血再灌注(I/R)损伤细胞模型氧糖剥夺/再灌注(OGD/R)诱导的HT22细胞损伤的保护作用,重点关注SIRT1/FOXO1信号通路。方法:采用商品化试剂盒检测细胞毒性、细胞凋亡、活性氧(ROS)生成、丙二醛(MDA)含量、4-羟基壬烯酸(4-HNE)水平、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性。采用酶联免疫吸附试验(ELISA)检测炎症细胞因子水平。Western blot检测蛋白表达情况。结果:柚皮素可显著改善OGD/ r诱导的HT22细胞毒性和凋亡。同时,柚皮素促进了OGD/ r作用下HT22细胞中SIRT1和fox01蛋白的表达。此外,柚皮素还能减弱OGD/ r诱导的细胞毒性、细胞凋亡、氧化应激(ROS、MDA和4-HNE水平升高,SOD、GSH-Px和CAT活性降低)和炎症反应(肿瘤坏死因子-α、白细胞间素[IL]-1β和IL-6水平升高,IL-10水平降低),这些炎症反应是通过抑制SIRT1- sirna诱导的SIRT1/FOXO1信号通路而被阻断的。结论:柚皮素通过促进SIRT1/FOXO1信号通路,通过抗氧化和抗炎活性保护HT22细胞免受OGD/R损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.90
自引率
9.10%
发文量
60
审稿时长
3-8 weeks
期刊介绍: Information not localized
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