Correlation between plasma long non-coding RNA MEG-3 and inflammatory cytokines in patients with diabetic nephropathy.

IF 1.1 4区医学 Q3 UROLOGY & NEPHROLOGY

Clinical nephrology Pub Date : 2023-08-01 DOI:10.5414/CN110996

Xingrong Guo, Meishe Gan, Lianji Zhou, Qingchen Wei, Zheng Li, Zuojie Luo, Hua Wei

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Abstract

To identify the correlation between the plasma long non-coding RNA maternally expressed gene 3 (lncRNA MEG-3) and inflammatory cytokines in patients with diabetic nephropathy (DN) and search for a potential index for the diagnosis of DN. Quantitative real-time PCR (qPCR) was used to assess lncRNA MEG-3 expression. The levels of plasma cytokines were detected via enzyme-linked immunosorbent assay (-ELISA). 20 patients with type 2 diabetes (T2DM) and DN (DM+DN+ group), 19 patients with T2DM (DM+DN- group), and 17 healthy subjects (DM-DN- group) were finally enrolled. The expression of lncRNA MEG-3 was significantly upregulated in the DM+DN+ group compared to the DM+DN- group (p < 0.05) and the DM-DN- group (p < 0.001). Pearson's correlation analysis showed a positive correlation of lncRNA MEG-3 levels with cystatin C (Cys-C) (r = 0.468, p < 0.05), albumin-creatinine ratio (ACR) (r = 0.532, p < 0.05), and creatinine (Cr) (r = 0.468, p < 0.05), and a negative correlation with estimated glomerular filtration rate (eGFR) (r = -0.674, p < 0.01). Furthermore, the expression level of plasma lncRNA MEG-3 had a significantly positive correlation with the level of interleukin 1β (IL-1β) (r = 0.524, p < 0.05) and interleukin 18 (IL-18) (r = 0.230, p < 0.05). Binary regression analysis showed that lncRNA MEG-3 was a risk factor for DN with odds ration (OR) value of 1.71 (p < 0.05). The area under receiver operation characteristic (ROC) curve (AUC) of DN identified by lncRNA MEG-3 was 0.724. LncRNA MEG-3 was highly expressed in DN patients and showed a positive correlation with IL-1β, IL-18, ACR, Cys-C, and Cr.

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糖尿病肾病患者血浆长非编码RNA MEG-3与炎性细胞因子的相关性。

探讨糖尿病肾病（DN）患者血浆长非编码RNA母细胞表达基因3（lncRNA-MEG-3）与炎性细胞因子的相关性，寻找诊断DN的潜在指标。定量实时PCR（qPCR）用于评估lncRNA MEG-3的表达。通过酶联免疫吸附试验（-ELISA）检测血浆细胞因子水平。最终纳入20名2型糖尿病（T2DM）和DN患者（DM+DN+组）、19名T2DM患者（DM+TN-组）和17名健康受试者（DM-DN-组）。与DM+DN-组（p<0.05）和DM-DN-组（p<0.001）相比，DM+DN+组的lncRNA MEG-3表达显著上调。Pearson相关分析显示，lncRNA MEG-3水平与胱抑素C（Cys-C）（r=0.468，p<0.05）、白蛋白-肌酸酐比（ACR）（r=0.532，p<0.05）和肌酸酐（Cr）（r=0.468，p>0.05）呈正相关，与肾小球滤过率（eGFR）呈负相关（r=-0.674，血浆lncRNA-MEG-3的表达水平与白细胞介素1β（IL-1β）（r=0.524，p<0.05）和白细胞介素18（IL-18）水平（r=0.230，p<0.05）呈显著正相关。二元回归分析表明，lncRNA-MEG-3是DN的危险因素，比值比（OR）值为1.71（p<0.05）经lncRNA MEG-3鉴定为0.724。LncRNA MEG-3在DN患者中高表达，并与IL-1β、IL-18、ACR、Cys-C和Cr呈正相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical nephrology 医学-泌尿学与肾脏学

CiteScore

2.10

自引率

9.10%

发文量

138

审稿时长

4-8 weeks

期刊介绍： Clinical Nephrology appears monthly and publishes manuscripts containing original material with emphasis on the following topics: prophylaxis, pathophysiology, immunology, diagnosis, therapy, experimental approaches and dialysis and transplantation.