A Study of Antibacterial Effect of Nigella sativa Seed Extracts on Bacterial Isolates from Cases of Wound Infection.

Q3 Pharmacology, Toxicology and Pharmaceutics
Boinu Babu, Pooja Rao, Ethel Suman, Jeppu Udayalaxmi
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Abstract

Background: With an increasing trend of pathogenic bacteria developing resistance to the existing drugs, there is a need for newer therapeutic measures. Nigella sativa seeds and oil have been used for decades as Ayurveda, Unani Tibb and other forms of traditional medicine for various disorders. Thymoquinone is one of the active components of the N. sativa seeds.

Objective: The present study determines the antibacterial effect of crude methanolic extract N. sativa seeds and thymoquinone against bacteria causing wound infection.

Methods: Samples obtained from cases of wound infection received at a Microbiology laboratory attached to a tertiary care hospital over a period of six months were included in the study. The antibacterial effect of crude methanolic extract of N. sativa seeds was determined by the Punch Well method. The minimum inhibitory concentration (MIC) of thymoquinone against bacteria isolated from cases of wound infection was determined by the Micro Broth Dilution technique.

Results: A total of 60 isolates were collected from 60 samples of wound infection. By the Punch Well method, Staphylococcus aureus showed varying zones of inhibition whereas all gram-negative bacilli and Enterococcus faecalis did not show any zone of inhibition. Thymoquinone showed good antibacterial activity against S. aureus with MIC values ranging from 2-8μg/ml for most of the isolates. Uniformly, MIC of thymoquinone against all gram-negative bacilli and E. faecalis was >128μg/ml, p<0.001. It was found that methicillin-resistant S. aureus (MRSA) isolates showed higher MIC than methicillin sensitive S. aureus (MSSA) isolates p<0.05.

Conclusion: Antibacterial activity of thymoquinone was very good against S. aureus but showed limited activity against Enterobacteriaceae members and E. faecalis isolated from patients with wound infection. Thymoquinone may be considered a potential antibacterial agent against wound infection caused by S. aureus.

黑草种子提取物对伤口感染分离菌抑菌作用的研究。
背景:随着病原菌对现有药物产生耐药性的趋势日益增加,需要新的治疗措施。几十年来,黑草种子和油一直被用作阿育吠陀、乌尼提布和其他形式的传统药物,用于治疗各种疾病。百里醌是sativa种子的有效成分之一。目的:研究芥蓝种子粗甲醇提取物和百里醌对伤口感染细菌的抑菌作用。方法:从一家三级医院附属微生物实验室接收的伤口感染病例中获得的样本在六个月内被纳入研究。采用冲孔法测定了芥蓝种子粗甲醇提取物的抑菌效果。采用微肉汤稀释法测定百里醌对伤口感染病原菌的最低抑菌浓度。结果:从60例伤口感染病例中共分离到60株细菌。Punch Well法对金黄色葡萄球菌有不同的抑制区,而对革兰氏阴性杆菌和粪肠球菌均无抑制区。百里醌对金黄色葡萄球菌具有良好的抑菌活性,多数菌株的MIC值在2 ~ 8μg/ml之间。结论:百里醌对金黄色葡萄球菌具有良好的抑菌活性,但对伤口感染患者分离的肠杆菌科细菌和粪肠杆菌的抑菌活性有限。百里醌可能被认为是一种潜在的抗金黄色葡萄球菌引起的伤口感染的抗菌剂。
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来源期刊
Infectious disorders drug targets
Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
3.10
自引率
0.00%
发文量
123
期刊介绍: Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.
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