Human Mesenchymal Stem Cells Improve Angiogenesis and Bone Formation in Severed Finger Rats through SIRT1/Nrf2 Signaling.

IF 2.1 4区医学 Q4 CELL & TISSUE ENGINEERING

Current stem cell research & therapy Pub Date : 2024-01-01 DOI:10.2174/1574888X18666230512112735

Hao Wu, Weixue Sun, Gong Cheng, Mingdi Zheng, Yuchi Zhao, Zhilin Cao

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引用次数: 0

Abstract

Background: This study employed a severed finger rat model to analyze the effects of human mesenchymal stem cells (MSCs) on angiogenesis, inflammatory response, apoptosis, and oxidative stress, to evaluate the possible mechanism of the repair effect of MSCs on severed finger (SF) rats.

Methods: Sixty Sprague-Dawley (SD) rats were categorized into five groups (n = 12). The pathological changes of severed finger tissues were investigated by Hematoxylin and eosin (H&E) staining on day 14 after the rats were sacrificed. The levels of inflammatory factors and oxidative stress factors were detected by ELISA. Terminal Deoxynucleotidyl Transferase (TdT) dUTP Nick End Labeling (TUNEL) was employed to assess the apoptosis of chondrocytes in severed finger tissues. The expression of osteocalcin (OCN), osteopontin (OPN), Collagen I (Col-1), and CD31 were detected by immunohistochemistry or immunofluorescence assay, respectively. The expression levels of related proteins were determined by western blot.

Result: Our study presented evidence that MSCs treatment improved pathological changes of skin and bone tissue, diminished the inflammatory response, prevented oxidative stress injury, suppressed chondrocyte apoptosis, and promoted angiogenesis, and bone formation compared to the model group. In addition, EX527 treatment attenuated the effect of MSCs, SRT1720 and ML385 co-treatment also attenuated the effect of MSCs. Importantly, the MSCs treatment increased the expression of Sirtuin 1(SIRT1)/Nuclear factor erythroid2-related factor 2(Nrf2) relate proteins.

Conclusion: Our study indicated that the mechanism of the effect of MSCs on a severed finger was related to the SIRT1/ Nrf2 signaling pathway.

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人类间充质干细胞通过 SIRT1/Nrf2 信号改善断指大鼠的血管生成和骨骼形成

背景：本研究采用断指大鼠模型，分析人间充质干细胞（MSCs）对血管生成、炎症反应、细胞凋亡和氧化应激的影响，评价间充质干细胞对断指大鼠修复作用的可能机制：方法：将 60 只 Sprague-Dawley (SD) 大鼠分为 5 组（n = 12）。方法：将 60 只 Sprague-Dawley (SD) 断指大鼠分为 5 组（n = 12），在大鼠处死后第 14 天用苏木精和伊红（H&E）染色法检测断指组织的病理变化。用酶联免疫吸附法检测炎症因子和氧化应激因子的水平。末端脱氧核苷酸转移酶（TdT）dUTP尼克末端标记（TUNEL）用于评估断指组织中软骨细胞的凋亡。骨钙素（OCN）、骨生成素（OPN）、胶原蛋白 I（Col-1）和 CD31 的表达分别通过免疫组化或免疫荧光检测。相关蛋白的表达水平由 Western 印迹法测定：结果：我们的研究证明，与模型组相比，间充质干细胞治疗可改善皮肤和骨组织的病理改变，减轻炎症反应，防止氧化应激损伤，抑制软骨细胞凋亡，促进血管生成和骨形成。此外，EX527 治疗可减轻间充质干细胞的作用，SRT1720 和 ML385 联合治疗也可减轻间充质干细胞的作用。重要的是，间充质干细胞治疗增加了Sirtuin 1（SIRT1）/核因子红细胞相关因子2（Nrf2）相关蛋白的表达：我们的研究表明，间充质干细胞对断指的作用机制与 SIRT1/ Nrf2 信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current stem cell research & therapy CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

4.20

自引率

3.70%

发文量

197

审稿时长

>12 weeks

期刊介绍： Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.