[Features of aging replicative and biochemical aspects among females with non-iatrogenic hypergonadotropic hypogonadism].

Q4 Medicine

Problemy endokrinologii Pub Date : 2023-06-30 DOI:10.14341/probl13295

R K Mikheev, E N Andreeva, O R Grigoryan, E V Sheremetyeva, Yu S Absatarova, N N Volevodz, E V Loginova

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引用次数: 0

Abstract

Background: Estrogenic deficiency is the basic condition of human ageing that leads to hypergonadotropic hypogonadism. The existence of correlation between hypergonadotropic hypogonadism, replicative (leukocyte telomere length) and biochemical data is widely supposed among females with physiological (menopausal) and pathological (primary ovarian insufficiency) estrogenic deficiency is not unreasonable.

Aim: To evaluate features of replicative (telomere length) and biochemical (metabolic syndrome) ageing markers among females with physiological (menopausal) and pathological (primary ovarian insufficiency) estrogenic deficiency.

Materials and methods: Research has been provided in collaboration between Endocrinology Research Centre of the Russian Ministry of Health and Lomonosov Moscow State University Medical Research and Educational Centre in the period since 10.01.2021 until 01.08.2022.110 females (20-75y.o.) have participated in the present research.Group 1: 26 females receiving menopausal hormonal therapy (MHT) ≥ 5 years with 0,5; 1; 2 mg estrogenic component.Group 2: 27 females in physiological menopause without MHTGroup 3: 33 females with primary ovarian insufficiency and receiving sex-steroid replacement therapy.Group 4: 24 healthy reproductive age females without sex-steroid replacement therapy.Patients have undergone laboratory genetic (leucocyte telomere length), biochemical analyses.DNA extraction - with Qiagen DNA blood mini kit (Germany). Biological material was cito conserved with Ficoll solution. Leukocyte telomere length - with real-time polymerase chain reaction PCR (Flow-fish).Soft program IBM SPSS Statistics (version 26,0 for Windows) has been used for statical analysis.

Results: 1.Menopausal females receiving MHT were inclined to highest HDL-P levels (p<0,006).2.Females with primary ovarian insufficiency were inclined to relatively highest serum creatinine level (p<0,001).3.Reproductive age females had relatively highest telomere length (p<0,001).4.FSH level correlates negatively and moderately (ρ= - 0,434) leukocyte telomere length (р<0,001) among females.

Conclusion: Females with premature ovarian insufficiency are most sensible to ageing due to features of replicative and biochemical markers.

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[非医源性促性腺激素亢进性性腺功能减退症女性的衰老、复制和生化特征]。

背景:雌激素缺乏是人类衰老的基本条件，导致促性腺激素亢进性性腺功能减退。在生理性(绝经期)和病理性(原发性卵巢功能不全)雌激素缺乏的女性中，促性腺功能亢进与复制(白细胞端粒长度)和生化数据之间存在相关性，这并非不合理。目的:探讨生理性(绝经期)和病理性(原发性卵巢功能不全)雌激素缺乏女性的生殖(端粒长度)和生化(代谢综合征)衰老标志物的特征。材料和方法:自2021年1月10日至2022.8月1日期间，俄罗斯卫生部内分泌学研究中心和罗蒙诺索夫莫斯科国立大学医学研究和教育中心合作开展了研究，共有110名女性(20-75岁)参加了本研究。第一组:26名接受绝经期激素治疗(MHT)≥5年的女性，分别为0,5;1;2毫克雌激素成分。第2组:无mht27例生理性绝经女性，第3组:33例原发性卵巢功能不全接受性类固醇替代治疗的女性。第4组:24名未接受性类固醇替代治疗的健康育龄女性。患者进行了实验室遗传(白细胞端粒长度)、生化分析。DNA提取-使用Qiagen DNA血液迷你试剂盒(德国)。用Ficoll溶液保存生物材料。白细胞端粒长度-实时聚合酶链反应PCR (Flow-fish)。软件程序IBM SPSS Statistics (version 26,0 for Windows)已用于统计分析。结果:1。绝经期接受MHT的女性倾向于HDL-P水平最高(p< 0.006)。原发性卵巢功能不全的女性血清肌酐水平相对较高(p< 0.001)。育龄女性端粒长度相对最高(p < 0.01)。在女性中，FSH水平与白细胞端粒长度(ρ= - 0,434)呈中度负相关(ρ= - 0,434)。结论:卵巢功能不全的女性在生殖和生化指标上对衰老最敏感。

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来源期刊

Problemy endokrinologii Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Since 1955 the “Problems of Endocrinology” (or “Problemy Endocrinologii”) Journal publishes timely articles, balancing both clinical and experimental research, case reports, reviews and lectures on pressing problems of endocrinology. The Journal is aimed to the most topical issues of endocrinology: to chemical structure, biosynthesis and metabolism of hormones, the mechanism of their action at cellular and molecular level; pathogenesis and to clinic of the endocrine diseases, new methods of their diagnostics and treatment. The Journal: features original national and foreign research articles, reflecting world endocrinology development; issues thematic editions on specific areas; publishes chronicle of major international congress sessions and workshops on endocrinology, as well as state-of-the-art guidelines; is intended for scientists, endocrinologists diabetologists and specialists of allied trade, general practitioners, family physicians and pediatrics.