Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia.

IF 11.3 1区 医学 Q1 Medicine
Gyu Tae Park, Jae Kyung Lim, Eun-Bae Choi, Mi-Ju Lim, Bo-Young Yun, Dae Kyoung Kim, Jung Won Yoon, Yoon Gi Hong, Jae Hoon Chang, Seong Hwan Bae, Jung Yong Ahn, Jae Ho Kim
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引用次数: 0

Abstract

Background: Adipose tissue-derived microvascular fragments are functional vessel segments derived from arterioles, capillaries, and veins. Microvascular fragments can be used as vascularization units in regenerative medicine and tissue engineering containing microvascular networks. However, the in vivo therapeutic and vascularization properties of human microvascular fragments have not been investigated.

Methods: In this study, we isolated microvascular fragments, stromal vascular fractions, and mesenchymal stem cells from human lipoaspirate and studied their therapeutic efficacy and in vivo vasculogenic activity in a murine model of hindlimb ischemia. In addition, in vivo angiogenic activity and engraftment of microvascular fragments into blood vessels were measured using Matrigel plug assay.

Results: Both microvascular fragments and stromal vascular fractions contain not only mesenchymal stem cells but also endothelial progenitor cells. In a Matrigel plug assay, microvascular fragments increased the number of blood vessels containing red blood cells more than mesenchymal stem cells and stromal vascular fractions did. The engraftment of the microvascular fragments transplanted in blood vessels within the Matrigel plug significantly increased compared to the engraftment of mesenchymal stem cells and stromal vascular fractions. Moreover, intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis compared to that of mesenchymal stem cells or stromal vascular fractions. Furthermore, transplanted microvascular fragments formed new blood vessels in ischemic limbs.

Conclusions: These results suggest that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering. Adipose tissue-derived microvascular fragments are vascularization units in regenerative medicine and tissue engineering containing microvascular networks. Intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis. The present study suggests that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering.

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脂肪组织来源的微血管碎片移植促进重症肢体缺血的治疗。
背景:脂肪组织衍生的微血管碎片是源自小动脉、毛细血管和静脉的功能性血管片段。含有微血管网络的微血管碎片可作为再生医学和组织工程中的血管化单元。然而,人体微血管碎片的体内治疗和血管化特性尚未被研究。方法:从人抽脂液中分离微血管片段、间质血管片段和间充质干细胞,在小鼠后肢缺血模型中研究其治疗效果和体内血管生成活性。此外,采用Matrigel塞法测定了体内血管生成活性和微血管碎片植入血管的情况。结果:微血管碎片和间质血管碎片均含有间充质干细胞和内皮祖细胞。在Matrigel塞试验中,微血管碎片比间充质干细胞和基质血管碎片更能增加含有红细胞的血管数量。与间充质干细胞和间质血管组分相比,移植到血管内的微血管碎片的植入明显增加。此外,与间充质干细胞或间质血管组分相比,肌内注射微血管碎片可显著增加缺血后肢的血流量,减轻组织坏死。此外,移植的微血管碎片在缺血肢体中形成了新的血管。结论:微血管碎片在体内具有较高的植入效率和血管生成活性,在缺血性疾病的治疗和组织工程中具有重要的应用价值。脂肪组织衍生的微血管碎片是再生医学和组织工程中含有微血管网络的血管化单元。肌内注射微血管碎片可明显增加缺血后肢的血流量,减轻组织坏死。本研究表明,微血管碎片在体内具有较高的植入效率和血管生成活性,在缺血性疾病的治疗和组织工程中具有重要的应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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