{"title":"Isolation of <i>Bacillus amyloliquefaciens</i> M13-RW0 from Soil and Evaluation of its Antifungal Activity.","authors":"Shirin Naghdifar, Mahboobeh Madani, Pegah Shakib","doi":"10.2174/1570163820666230419090347","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The frequency of observed invasive Aspergillosis has increased in recent years. Infection with other molds happens but does not lead to a large proportion of invasive infections. The present study aims to isolate Bacillus amyloliquefaciens M13-RW0 from soil and evaluate its antifungal effects against some saprophytic fungi, such as Aspergillus niger, Aspergillus flavus, and Mucor hiemalis.</p><p><strong>Materials and methods: </strong>In this research, a total of 150 samples (from the soil, air, and surfaces) were prepared from different parts of Isfahan, Iran. Isolation and purification of growing bacteria were conducted using the nutrient agar medium. The inhibitory effects of 100 isolated bacteria were evaluated against the growth of A. niger, A. flavus, and M. hiemalis, 4 bacteria were isolated with inhibitory effects against the selected fungi, and consequently, one of the bacteria isolated from the soil samples was found to show the highest inhibition of fungal growth. Quantitative evaluation of the growth inhibitory effect was performed using linear culturing of fungal suspension (10<sup>4</sup> spore/ml) at distances of 5, 10, 15, 20, 25, and 30mm from bacterial isolate (0.5 McFarland) on Sabouraud Dextrose Agar (SDA) medium. Results were checked 24, 48, 72, and 96 hours later. The bacterial isolate with the most inhibitory effect was identified by phenotypic and molecular tests.</p><p><strong>Results: </strong>According to the results, among the 4 inhibitory bacterial isolates, Bacillus amyloliquefaciens strain M13-RW01, isolated from the soil samples, was identified as the bacterium with the most significant potential antifungal activity. The strong inhibitory effect was revealed after 48 hours for all distances of 15mm and more between the fungi and the bacterium.</p><p><strong>Conclusion: </strong>The identified bacterium could not only be considered an inhibitor bacterium against saprophytic fungi but could also be put forward to help produce new antifungal drugs for controlling fungal diseases.</p>","PeriodicalId":10858,"journal":{"name":"Current drug discovery technologies","volume":"20 4","pages":"e190423216028"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current drug discovery technologies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1570163820666230419090347","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The frequency of observed invasive Aspergillosis has increased in recent years. Infection with other molds happens but does not lead to a large proportion of invasive infections. The present study aims to isolate Bacillus amyloliquefaciens M13-RW0 from soil and evaluate its antifungal effects against some saprophytic fungi, such as Aspergillus niger, Aspergillus flavus, and Mucor hiemalis.
Materials and methods: In this research, a total of 150 samples (from the soil, air, and surfaces) were prepared from different parts of Isfahan, Iran. Isolation and purification of growing bacteria were conducted using the nutrient agar medium. The inhibitory effects of 100 isolated bacteria were evaluated against the growth of A. niger, A. flavus, and M. hiemalis, 4 bacteria were isolated with inhibitory effects against the selected fungi, and consequently, one of the bacteria isolated from the soil samples was found to show the highest inhibition of fungal growth. Quantitative evaluation of the growth inhibitory effect was performed using linear culturing of fungal suspension (104 spore/ml) at distances of 5, 10, 15, 20, 25, and 30mm from bacterial isolate (0.5 McFarland) on Sabouraud Dextrose Agar (SDA) medium. Results were checked 24, 48, 72, and 96 hours later. The bacterial isolate with the most inhibitory effect was identified by phenotypic and molecular tests.
Results: According to the results, among the 4 inhibitory bacterial isolates, Bacillus amyloliquefaciens strain M13-RW01, isolated from the soil samples, was identified as the bacterium with the most significant potential antifungal activity. The strong inhibitory effect was revealed after 48 hours for all distances of 15mm and more between the fungi and the bacterium.
Conclusion: The identified bacterium could not only be considered an inhibitor bacterium against saprophytic fungi but could also be put forward to help produce new antifungal drugs for controlling fungal diseases.
背景:近年来侵袭性曲霉病的发病率有所上升。其他霉菌的感染会发生，但不会导致很大比例的侵袭性感染。本研究旨在从土壤中分离解淀粉芽孢杆菌M13-RW0，并评价其对一些腐生真菌，如黑曲霉、黄曲霉和毛霉的抑菌作用。材料和方法:在本研究中，从伊朗伊斯法罕的不同地区共制备了150个样品(来自土壤，空气和表面)。利用营养琼脂培养基对生长菌进行分离纯化。对100株分离细菌对黑曲霉、黄曲霉和黑曲霉生长的抑制作用进行了评价，分离到4株对所选真菌有抑制作用的细菌，结果发现从土壤样品中分离到的一株细菌对真菌生长的抑制作用最大。在Sabouraud Dextrose Agar (SDA)培养基上，用真菌悬浮液(104孢子/ml)在距离为5、10、15、20、25和30mm的地方进行线性培养，定量评估生长抑制效果。24小时、48小时、72小时和96小时后检查结果。通过表型和分子检测鉴定出抑菌效果最好的分离菌。结果:4株抑菌菌株中，从土壤样品中分离的解淀粉芽孢杆菌菌株M13-RW01为抑菌活性最显著的菌株。48小时后，真菌与细菌之间的距离在15mm及以上均有较强的抑制作用。结论:该细菌不仅可作为腐生真菌的抑制菌，而且可用于开发新的抗真菌药物来控制真菌疾病。
期刊介绍：
Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.
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