Functional responsiveness of in vitro-aged human neutrophils

IF 3.7 4区 医学 Q2 CELL BIOLOGY
J. Morin-Genest, A. Saafane, D. Girard
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引用次数: 0

Abstract

Elimination of apoptotic neutrophils by macrophages is as a major step for the resolution of inflammation. However, the fate and the cellular functionality of neutrophils aged in the absence of macrophages are not well documented. Herein, freshly isolated human neutrophils were aged for several days in vitro and then stimulated with agonists for determining their cell responsiveness. In vitro-aged neutrophils were still able to generate reactive oxygen species after 48 h, exert phagocytosis after 72 h, and increase their adhesion onto a cell substratum after 48 h. These data demonstrate that a portion of neutrophils cultivated for several days in vitro are still able to exert biological functions. This opens the possibility that, during inflammation, neutrophils may still respond to agonists, a condition that is likely to occur in vivo when they are not efficiently eliminated by efferocytosis.

体外衰老人中性粒细胞的功能反应性
巨噬细胞清除凋亡中性粒细胞是解决炎症的重要步骤。然而,在没有巨噬细胞的情况下,中性粒细胞衰老的命运和细胞功能并没有很好的记录。在这里,新鲜分离的人中性粒细胞在体外老化数天,然后用激动剂刺激以确定它们的细胞反应性。体外老化的中性粒细胞在48 h后仍能产生活性氧,72 h后仍能发挥吞噬作用,48 h后仍能增强对细胞基质的粘附。这些数据表明,部分体外培养数天的中性粒细胞仍能发挥生物学功能。这开启了一种可能性,在炎症期间,中性粒细胞可能仍然对激动剂有反应,这种情况很可能发生在体内,当它们不能被efferocytosis有效地消除时。
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来源期刊
Cellular immunology
Cellular immunology 生物-免疫学
CiteScore
8.20
自引率
2.30%
发文量
102
审稿时长
30 days
期刊介绍: Cellular Immunology publishes original investigations concerned with the immunological activities of cells in experimental or clinical situations. The scope of the journal encompasses the broad area of in vitro and in vivo studies of cellular immune responses. Purely clinical descriptive studies are not considered. Research Areas include: • Antigen receptor sites • Autoimmunity • Delayed-type hypersensitivity or cellular immunity • Immunologic deficiency states and their reconstitution • Immunologic surveillance and tumor immunity • Immunomodulation • Immunotherapy • Lymphokines and cytokines • Nonantibody immunity • Parasite immunology • Resistance to intracellular microbial and viral infection • Thymus and lymphocyte immunobiology • Transplantation immunology • Tumor immunity.
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