Fish Hacks: Hybridizing Stand-Alone Zebrafish System Plumbing and Pumps to Extend and Improve Function.

Abstract

One of the greatest expenses in running a zebrafish laboratory is the aquatic systems used for housing. These critical pieces of equipment are essential and incorporate components undergoing constant activity in pumping water, monitoring, dosing, and filtration. The systems available on the market are robust, yet ongoing activity eventually leads to the need for repair or replacement. Moreover, some systems are no longer commercially available, impairing the ability to service this critical infrastructure. In this study, we demonstrate a do it yourself (DIY) method to re-engineer an aquatic system's pumps and plumbing, which hybridizes a system no longer commercially available with components used by active vendors. This transition from a two external pump Aquatic Habitat/Pentair design to an individual submerged pump Aquaneering-like plan saves funds by expanding infrastructure longevity. Our hybridized configuration has been in uninterrupted use for >3 years, supporting zebrafish health and high fecundity.