Severe Anemia in Sprague-Dawley Rats After Roux-en-Y Gastric Bypass Surgery.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2023-06-01 Epub Date: 2023-05-31 DOI:10.30802/AALAS-CM-22-000074
Rachel L Griffin, Ashley N Varley, Andras Hajnal, Jennifer L Booth
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引用次数: 0

Abstract

Roux-en-Y gastric bypass (RYGB) surgery is one of the most commonly performed bariatric procedures for weight loss in humans. However, this procedure is not risk-free, and patients may experience complications that include small bowel obstruction, gastrointestinal bleeding, chronic diarrhea, ulcers, malnutrition, and anemia. In particular, anemia is a recognized long-term complication and can be severe. Rats have been used as a model to study the effects of gastric bypass surgeries. They can experience similar complications as people, but the development of severe anemia has not previously been reported in rats. We observed 2 cases of severe anemia in female Sprague-Dawley rats after RYGB surgery. These cases prompted us to further investigate the frequency and severity of anemia after RYGB in rats. Blood work and necropsies were performed on 9 additional female Sprague-Dawley rats (5 with RYGB, 4 with sham surgery). In these 9 rats, only one had signs of clinical anemia. These 3 anemic rats displayed moderate to severe pallor of the eyes and ears. Necropsy findings in anemic RYGB rats included pale internal organs and eccentric heart enlargement, which led to a significantly higher heart:body weight ratio in RYGB rats as compared with sham controls. Anemic rats had either a macrocytic normochromic anemia, consistent with vitamin B12 or folate deficiency, or microcytic hypochromic anemia, indicative of iron deficiency. Researchers who perform RYGB surgery in rats should be aware of the potential complication of severe anemia. Plans for the diagnosis and management of this complication and the development of criteria for humane endpoints for severe anemia are recommended as a refinement to these studies.

Roux-en-Y胃旁路手术后Sprague-Dawley大鼠的严重贫血。
Roux-en-Y胃旁路手术(RYGB)是人类最常用的减肥手术之一。然而,这一过程并非没有风险,患者可能会出现并发症,包括小肠梗阻、胃肠道出血、慢性腹泻、溃疡、营养不良和贫血。特别是,贫血是一种公认的长期并发症,可能很严重。以大鼠为模型研究胃分流术的效果。它们可以经历与人类相似的并发症,但以前没有在老鼠身上报道过严重贫血的发展。我们观察到2例雌性Sprague-Dawley大鼠RYGB手术后严重贫血。这些病例促使我们进一步研究大鼠RYGB后贫血的频率和严重程度。另外对9只雌性Sprague-Dawley大鼠进行了血液检查和尸检(5只采用RYGB, 4只采用假手术)。在这9只大鼠中,只有一只有临床贫血的迹象。这3只贫血大鼠的眼睛和耳朵出现中度至重度苍白。贫血RYGB大鼠的尸检结果包括内脏苍白和心脏偏心增大,导致RYGB大鼠的心重比明显高于假对照组。贫血大鼠要么是大细胞正色性贫血,与维生素B12或叶酸缺乏一致,要么是小细胞低色性贫血,表明缺铁。在大鼠身上进行RYGB手术的研究人员应该意识到严重贫血的潜在并发症。建议制定诊断和管理该并发症的计划,并制定严重贫血的人道终点标准,作为这些研究的改进。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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