Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/7651_2023_494

Katherine Vandal, Savana Biondic, Jesica Canizo, Sophie Petropoulos

引用次数: 0

Abstract

Single-cell genomics allow the characterization and quantification of molecular heterogeneity from a wide variety of tissues. Here, we describe the manual dissociation and collection of single cells, a method adapted for the characterization of precious small tissues like preimplantation embryos. We also describe the acquisition of mouse embryos by flushing of the oviducts. The cells can then be used in multiple sequencing protocols, for example, Smart-seq2, Smart-seq3, smallseq, and scBSseq.

查看原文本刊更多论文

手动分离哺乳动物植入前胚胎以进行单细胞基因组学研究。

单细胞基因组学可以表征和量化各种组织的分子异质性。在此，我们介绍了手动分离和收集单细胞的方法，这种方法适用于对植入前胚胎等珍贵的小组织进行表征。我们还介绍了通过冲洗输卵管获取小鼠胚胎的方法。然后，这些细胞可用于多种测序方案，例如 Smart-seq2、Smart-seq3、smallseq 和 scBSseq。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.