Usefulness of measuring fast valley height rate using high-performance liquid chromatography for variant hemoglobin, Hb A2-Niigata.

IF 1.3 Q4 ENDOCRINOLOGY & METABOLISM

Diabetology International Pub Date : 2023-01-25 eCollection Date: 2023-04-01 DOI:10.1007/s13340-023-00617-1

Mika Ito, Masataka Shikata, Masafumi Koga

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引用次数: 0

Abstract

We have reported cases of hemoglobin (Hb) A2-Niigata (δ1Val → Ala) with δ chain variant Hb and falsely high HbA1c levels. In subjects with Hb A2-Niigata, shoulder-shaped anomalous peak was detected immediately before the HbA1c peaks using high-performance liquid chromatography (HPLC; HLC-723 G9). The phenomenon where the valley between peaks of LA1c+ , a fraction containing labile HbA1c, and HbA1c fraction [fast valley (FV)] became shallow owing to the anomalous peak was observed. In this study, we attempted to quantify the height index of the FV. We assumed that the index FV height rate (FVHR) was useful for screening Hb A2-Niigata. Five subjects with Hb A2-Niigata were compared with the control group of 50 subjects without diabetes and 50 with diabetes. Various indices were measured using HPLC chromatograms, and FVHR was calculated based on these indices. FVHR in subjects with Hb A2-Niigata was significantly higher than that in subjects without and with diabetes (all P < 0.001). Furthermore, when the cutoff value of FVHR was set at 23-30%, Hb A2-Niigata could be diagnosed with high sensitivity and specificity (both 100%). Hb A2-Niigata can be screened using FVHR with high sensitivity and specificity. FVHR might be also useful for screening other variant hemoglobins with abnormal HPLC chromatograph.

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使用高效液相色谱法测量变异血红蛋白 Hb A2-Niigata 的快速谷高率的实用性。

我们曾报告过血红蛋白（Hb）A2-Niigata（δ1Val → Ala）δ链变异型 Hb 和假性高 HbA1c 水平的病例。在 Hb A2-Niigata 患者中，使用高效液相色谱法（HPLC；HLC-723 G9）可在 HbA1c 峰之前检测到肩形异常峰。由于该异常峰的存在，我们观察到含有易变 HbA1c 的 LA1c+ 部分与 HbA1c 部分的峰间谷[快速谷（FV）]变浅的现象。在本研究中，我们尝试量化 FV 的高度指数。我们假设 FV 高度指数（FVHR）可用于筛查 Hb A2-新泻。我们将五名患有 Hb A2-Niigata 的受试者与对照组的 50 名非糖尿病受试者和 50 名糖尿病受试者进行了比较。使用高效液相色谱法测量了各种指数，并根据这些指数计算出 FVHR。患有 Hb A2-Niigata 的受试者的 FVHR 明显高于无糖尿病和有糖尿病的受试者（均为 P 0.001）。此外，当 FVHR 的临界值设定为 23-30% 时，Hb A2-Niigata 的诊断灵敏度和特异性都很高（均为 100%）。使用 FVHR 筛查 Hb A2-Niigata 的灵敏度和特异性都很高。FVHR 也可用于筛查其他 HPLC 色谱异常的变异血红蛋白。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diabetology International ENDOCRINOLOGY & METABOLISM-

CiteScore

3.90

自引率

4.50%

发文量

期刊介绍： Diabetology International, the official journal of the Japan Diabetes Society, publishes original research articles about experimental research and clinical studies in diabetes and related areas. The journal also presents editorials, reviews, commentaries, reports of expert committees, and case reports on any aspect of diabetes. Diabetology International welcomes submissions from researchers, clinicians, and health professionals throughout the world who are interested in research, treatment, and care of patients with diabetes. All manuscripts are peer-reviewed to assure that high-quality information in the field of diabetes is made available to readers. Manuscripts are reviewed with due respect for the author''s confidentiality. At the same time, reviewers also have rights to confidentiality, which are respected by the editors. The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.