Evaluation of Tamarix nilotica Fractions in Combating Candida albicans Infections.

IF 4.2 2区医学 Q1 INFECTIOUS DISEASES

Expert Review of Anti-infective Therapy Pub Date : 2024-04-01 Epub Date: 2023-07-04 DOI:10.1080/14787210.2023.2232112

Ehssan Moglad, Engy Elekhnawy, Walaa A Negm, Fatma A Mokhtar, Reem Binsuwaidan, Nashwah G M Attallah, Eman Ahmed, Sameh Magdeldin, Omnia Momtaz Al-Fakhrany

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引用次数: 0

Abstract

Objectives: Evaluation of the antifungal properties of Tamarix nilotica fractions against Candida albicans clinical isolates.

Methods: The in vitro antifungal potential was evaluated by agar well diffusion and broth microdilution methods. The antibiofilm potential was assessed by crystal violet, scanning electron microscopy (SEM), and qRT-PCR. The in vivo antifungal activity was evaluated by determining the burden in the lung tissues of infected mice, histopathological, immunohistochemical studies, and ELISA.

Results: Both the dichloromethane (DCM) and ethyl acetate (EtOAc) fractions had minimum inhibitory concentration (MIC) values of 64-256 and 128-1024 μg/mL, respectively. SEM examination showed that the DCM fraction decreased the biofilm formation capacity of the treated isolates. A significant decline in biofilm gene expression was observed in 33.33% of the DCM-treated isolates. A considerable decline in the CFU/g lung count in infected mice was observed, and histopathological examinations revealed that the DCM fraction maintained the lung tissue architecture. Immunohistochemical investigations indicated that the DCM fraction significantly (p < 0.05) decreased the expression of pro-inflammatory and inflammatory cytokines (TNF-α, NF-kB, COX-2, IL-6, and IL-1β) in the immunostained lung sections. The phytochemical profiling of DCM and EtOAc fractions was performed using Liquid chromatography-mass spectrometry (LC-ESI-MS/MS).

Conclusion: T. nilotica DCM fraction could be a significant source of natural products with antifungal activity against C. albicans infections.

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评估柽柳提取物在抗白色念珠菌感染中的作用。

目的方法：采用琼脂井扩散法和肉汤微稀释法评估玉竹馏分对白色念珠菌临床分离株的抗真菌特性：方法：采用琼脂井扩散法和肉汤微稀释法评估体外抗真菌潜力。抗生物膜的潜力通过水晶紫、扫描电子显微镜（SEM）和 qRT-PCR 进行评估。通过测定感染小鼠肺组织中的负担、组织病理学、免疫组织化学研究和酶联免疫吸附试验评估了体内抗真菌活性：结果：二氯甲烷（DCM）和乙酸乙酯（EtOAc）馏分的最低抑菌浓度（MIC）值分别为 64-256 μg/mL 和 128-1024 μg/mL。扫描电镜检查显示，DCM馏分降低了经处理的分离物的生物膜形成能力。在 33.33% 经 DCM 处理的分离物中观察到生物膜基因表达明显下降。受感染小鼠肺部的 CFU/g 数显著下降，组织病理学检查显示，DCM 部分保持了肺组织结构。免疫组化检查表明，DCM 部分显著（p 结论：DCM 部分对小鼠肺部组织结构的影响较小：黑木耳 DCM 部分可能是具有抗白僵菌感染活性的天然产品的重要来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Anti-infective Therapy 医学-药学

CiteScore

11.20

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Expert Review of Anti-Infective Therapy (ISSN 1478-7210) provides expert reviews on therapeutics and diagnostics in the treatment of infectious disease. Coverage includes antibiotics, drug resistance, drug therapy, infectious disease medicine, antibacterial, antimicrobial, antifungal and antiviral approaches, and diagnostic tests.