HPTLC Studies, in silico Docking Studies, and Pharmacological Evaluation of Elaeocarpus ganitrus as a Gastroprotective Agent.

Q3 Psychology
Anuj Kumar Sharma, Rajaneesh Kumar Chaudhary, Swetza Singh, Akash Ved, Karuna Shanker Shukla, Anita Singh, Manjul Pratap Singh, Mayank Kulshreshtha
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引用次数: 0

Abstract

Objectives: Elaeocarpus ganitrus, a member of the Eleocarpaceae family, is valued in Hinduism and Ayurveda, and is frequently used as a remedy for a variety of illnesses. The plant is reputed to treat a number of stomach issues. The purpose of the study was to produce high-quality scientific data regarding gastroprotective behavior, docking experiments with cholinergic receptors, and HPTLC (with lupeol and ursolic acid). To develop the mechanism of herbal extracts, in vitro anticholinergic and antihistaminic activities were evaluated. Different leaf extracts were treated with various reagents to determine the presence of various metabolites. An examination of the histopathology was conducted to determine the full impact of the extract.

Methods: Methanolic extract was chosen for HPTLC investigations after extraction with various solvents. A mobile phase of toluene, ethylacetate, and formic acid (8:2:0.1) was chosen. Molecular docking was utilized to examine how ursolic acid and lupeol are bound to cholinergic receptors (M3). Different extracts (aqueous and ethanolic) were tested for their ability to provide gastroprotection in Wistar rats at different doses (200 and 400 mg/kg).

Results: Phytochemical analysis of different extracts showed the presence of different primary and secondary metabolites. HPTLC data showed the presence of both standards. Docking studies exhibited very good interactions with the M3 receptor. Pharmacological studies revealed that extract-treated groups significantly reduced the ulcer index in all of the models mentioned above. The histopathological analysis clearly supports the biochemical studies, which were conducted utilizing various doses and found to be effective in a dose-dependent manner. The in vitro analysis proved that the abovementioned extracts may act as antagonists of acetylcholine and histamine.

Conclusion: The data obtained would be valuable for the production of the monograph of the plant and conducting concept-related clinical studies in the future. More investigation is required since the gathered scientific data may lead to new research opportunities.

作为胃保护剂的桔梗的 HPTLC 研究、硅学对接研究和药理评估
目的:Elaeocarpus ganitrus, eleaeocarpus ganitrus是elecarpacae家族的一员,在印度教和阿育吠陀中很有价值,经常被用来治疗各种疾病。这种植物被认为可以治疗许多胃部问题。该研究的目的是提供有关胃保护行为的高质量科学数据,与胆碱能受体对接实验,以及HPTLC(与鹿皮醇和熊果酸)。为了进一步研究中药提取物的作用机制,对其体外抗胆碱能和抗组胺活性进行了评价。用不同的试剂处理不同的叶提取物,以测定不同代谢物的存在。进行组织病理学检查以确定提取物的充分影响。方法:选择甲醇提取液,经不同溶剂提取后进行hplc检测。流动相为甲苯、乙酸乙酯和甲酸(8:2:1 . 0)。利用分子对接研究熊果酸和芦皮醇如何与胆碱能受体(M3)结合。在不同剂量(200和400 mg/kg)下,测试了不同提取物(水提物和乙醇提物)对Wistar大鼠胃保护的能力。结果:不同提取物的植物化学分析显示其主要代谢物和次级代谢物存在差异。HPTLC数据显示两种标准均存在。对接研究显示与M3受体有很好的相互作用。药理研究显示,提取物处理组显著降低上述所有模型的溃疡指数。组织病理学分析清楚地支持生化研究,这些研究是利用不同剂量进行的,并发现以剂量依赖的方式有效。体外实验证明,上述提取物具有乙酰胆碱和组胺拮抗剂的作用。结论:获得的数据对该植物专著的撰写和相关概念的临床研究具有一定的参考价值。由于收集到的科学数据可能会带来新的研究机会,因此需要进行更多的调查。
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来源期刊
Central nervous system agents in medicinal chemistry
Central nervous system agents in medicinal chemistry Psychology-Neuropsychology and Physiological Psychology
CiteScore
2.10
自引率
0.00%
发文量
21
期刊介绍: Central Nervous System Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new central nervous system agents. Containing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Central Nervous System Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.
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