Cytotoxic and anti-inflammatory effects of chitosan and hemostatic gelatin in oral cell culture.

Jessica J Narvaez-Flores, Gabriela Vilar-Pineda, Laura S Acosta-Torres, Rene Garcia-Contreras
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引用次数: 2

Abstract

Chitosan is a biopolymer with bactericidal/bacteriostatic effect, biocompatible and biodegradable. It has been used in tissue engineering to replace tissues partially or completely by releasing bioactive materials or influencing cell growth, usually in regenerative medicine and dentistry. The aim of this study was to evaluate the cytotoxic and anti-inflammatory effect of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF were isolated from patients. Cells were subcultured in DMEM. Chitosan was inoculated at different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) were placed directly in the presence of cells and incubated for 24 hours. Cell viability was determined by MTT method and mean cytotoxic concentration (CC50) was calculated from the dose-response curve. Anti-inflammatory effect was calculated from the in vitro gingivitis model induced with interleukin 1beta (IL-1β) in HGF and protein detection. The data were subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney tests. Experiments were performed in triplicate of three independent assays. Cell viability of HPC, HGF and MC3T3-E1 in contact with chitosan decreased significantly (p<0.05). The HPC were the most sensitive (CC50= 0.18%), followed by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory effect was reduced significantly in the gingivitis model. To conclude, chitosan induces moderate cytotoxic effects alone or with hemostatic gelatin at 0.19%, in dose-dependent manner, with anti-inflammatory effects on human gingival fibroblasts. The use of chitosan as a biomaterial can be an excellent choice for use in regenerative dentistry.

Abstract Image

壳聚糖和止血明胶在口腔细胞培养中的细胞毒和抗炎作用。
壳聚糖是一种具有杀菌/抑菌作用的生物聚合物,具有生物相容性和可生物降解性。它已被用于组织工程中,通过释放生物活性物质或影响细胞生长来部分或完全替代组织,通常用于再生医学和牙科。本研究的目的是评估壳聚糖单独或与止血明胶(Spongostand®)在人牙髓细胞(HPC)、人牙龈成纤维细胞(HGF)和小鼠前成骨细胞(MC3T3-E1, ATCC)培养中的细胞毒性和抗炎作用。从患者中分离HPC和HGF。细胞在DMEM中传代培养。接种不同浓度(0-0.5%)的壳聚糖,将壳聚糖浸透的止血明胶(0.19%)直接置于细胞存在下孵育24小时。MTT法测定细胞活力,量效曲线计算平均细胞毒浓度(CC50)。通过HGF中白细胞介素1β (IL-1β)诱导的牙龈炎体外模型及蛋白检测计算抗炎作用。这些数据进行了Shapiro-Wilk、Kruskal-Wallis和Mann-Whitney测试。实验是在三个独立的分析中重复进行的。壳聚糖使HPC、HGF和MC3T3-E1细胞活力显著降低(p
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