Knockdown of VASH2 Inhibits the Stemness and EMT Process by Regulating ZEB2 in Colorectal Cancer.

IF 2.1 4区医学 Q4 CELL & TISSUE ENGINEERING

Current stem cell research & therapy Pub Date : 2024-01-01 DOI:10.2174/1574888X18666230417084221

Paerhati Shayimu, Cheng Yin, Xiangyue Zeng, Rexida Jiapaer

{"title":"Knockdown of VASH2 Inhibits the Stemness and EMT Process by Regulating ZEB2 in Colorectal Cancer.","authors":"Paerhati Shayimu, Cheng Yin, Xiangyue Zeng, Rexida Jiapaer","doi":"10.2174/1574888X18666230417084221","DOIUrl":null,"url":null,"abstract":"Introduction: VASH2 is associated with the malignant progression of a variety of tumors, but the role and mechanism of VASH2 in colorectal cancer are still unclear.Methods: We analyzed the expression of VASH2 in colorectal cancer from the TCGA database and also analyzed the relationship between VASH2 expression and survival of colorectal cancer patients in the PrognoScan database. We verified the role of VASH2 in colorectal cancer through transfecting si-VASH2 into colorectal cancer cells and detecting cell viability by CCK8, cell migration by wound healing assay, and cell invasion by Transwell assay. ZEB2, Vimentin, and E- cadherin protein expression were examined by Western-Blot assay. Cell sphere-forming ability was determined by sphere formation assay, and we further confirmed the mechanism of VASH2 in colorectal cancer progression by rescue assays.Results: Colorectal cancer has a high expression of VASH2, and its expression is associated with a poorer patient survival rate. The vitality, migration, invasion, EMT, and tumor stemness of colorectal cancer cells were all decreased by VASH2 knockdown. These alternations were attenuated by ZEB2 overexpression.Conclusion: Our experiments confirmed that VASH2 affects colorectal cancer cell proliferation, migration, invasion, EMT, and seed bovine stemness by regulating ZEB2 expression.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"126-132"},"PeriodicalIF":2.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current stem cell research & therapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/1574888X18666230417084221","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: VASH2 is associated with the malignant progression of a variety of tumors, but the role and mechanism of VASH2 in colorectal cancer are still unclear.

Methods: We analyzed the expression of VASH2 in colorectal cancer from the TCGA database and also analyzed the relationship between VASH2 expression and survival of colorectal cancer patients in the PrognoScan database. We verified the role of VASH2 in colorectal cancer through transfecting si-VASH2 into colorectal cancer cells and detecting cell viability by CCK8, cell migration by wound healing assay, and cell invasion by Transwell assay. ZEB2, Vimentin, and E- cadherin protein expression were examined by Western-Blot assay. Cell sphere-forming ability was determined by sphere formation assay, and we further confirmed the mechanism of VASH2 in colorectal cancer progression by rescue assays.

Results: Colorectal cancer has a high expression of VASH2, and its expression is associated with a poorer patient survival rate. The vitality, migration, invasion, EMT, and tumor stemness of colorectal cancer cells were all decreased by VASH2 knockdown. These alternations were attenuated by ZEB2 overexpression.

Conclusion: Our experiments confirmed that VASH2 affects colorectal cancer cell proliferation, migration, invasion, EMT, and seed bovine stemness by regulating ZEB2 expression.

查看原文本刊更多论文

低表达VASH2通过调节ZEB2抑制结直肠癌的干细胞和EMT过程。

简介：VASH2与多种肿瘤的恶性进展有关，但其在癌症中的作用和机制尚不清楚。方法：我们从TCGA数据库中分析了VASH2在结直肠癌癌症中的表达，并在PrognoScan数据库中分析VASH2表达与结直肠癌癌症患者生存率的关系。我们通过将si-VASH2转染到结直肠癌癌症细胞中，并通过CCK8检测细胞活力，通过伤口愈合测定检测细胞迁移，通过Transwell测定检测细胞侵袭，验证了VASH2在结直肠癌癌症中的作用。用蛋白质印迹法检测ZEB2、波形蛋白和E-钙粘蛋白的表达。通过球体形成测定确定细胞球体形成能力，我们通过拯救测定进一步证实了VASH2在结直肠癌癌症进展中的机制。结果：癌症大肠癌组织中VASH2表达较高，其表达与患者生存率较低有关。VASH2的敲除使结直肠癌癌症细胞的活力、迁移、侵袭、EMT和肿瘤干度均下降。ZEB2过表达减弱了这些变化。结论：我们的实验证实，VASH2通过调节ZEB2的表达来影响癌症细胞的增殖、迁移、侵袭、EMT和种子牛干燥。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current stem cell research & therapy CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

4.20

自引率

3.70%

发文量

197

审稿时长

>12 weeks

期刊介绍： Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.