Stereological Evaluation of Rabbit Fetus Liver after Xenotransplantation of Human Wharton's Jelly-Derived Mesenchymal Stromal Cells.

IF 0.3 Q4 TRANSPLANTATION
Z Khodabandeh, L Rezaeian, M A Edalatmanesh, A Mogheiseh, N Tanideh, M Dianatpour, Sh Zare, H Bordbar, N Baghban, A Tamadon
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引用次数: 0

Abstract

Background: In-utero xenotransplantation of stem cells in abnormal fetuses effectively treats several genetic illnesses.

Objective: The current research aimed to evaluate structural and morphological alterations in the liver of rabbit fetuses following xenotransplantation of human Wharton's jelly-derived mesenchymal stromal cells (hWJ-MSCs), using a stereological technique.

Methods: All hWJ-MSCs were isolated from the human umbilical cord, and their authenticity was established by flowcytometry and differentiation. At gestational day 14, the rabbits were anesthetized, and hWJ-MSCs were injected into the uteri of 24 fetuses. Twenty-two fetuses were born successfully. Ten rabbit liver specimens were prepared from injected fetuses including; eight rabbits on day three following birth and two rabbits on the 21st post-natal day. The non-injected fetuses were considered positive controls. The livers of the control and hWJ-MSCs-treated rabbits were fixed, processed, stained, and examined through stereological approaches.

Results: In the hWJ-MSCs-treated group, the mean liver weight and volume increased by 42% and 78% compared to the control group. The total volume of the hepatocytes increased by 63%, and that of sinusoids by three folds in the treated rabbits. The total volume of the central veins increased by 70%. The total number corresponding to hepatocytes in the experimental group increased by 112% compared to the rabbits in the control. The total volume of the hepatocyte nuclei in the experimental group increased by 117% compared to the rabbits in the control.

Conclusion: After xenotransplantation of human MSCs, host tissue microenvironments (here, the rabbit liver) were altered and these included quantitative factors corresponding to the liver tissue and hepatocyte morphometric indices.

Abstract Image

人Wharton果冻间充质间质细胞异种移植后兔胎儿肝脏的体视学评价。
背景:异种胚胎干细胞在子宫内移植可有效治疗多种遗传性疾病。目的:本研究旨在利用体视学技术评估人华顿氏果冻源间充质间质细胞(hWJ-MSCs)异种移植后兔胎儿肝脏的结构和形态变化。方法:从人脐带中分离所有hWJ-MSCs,通过流式细胞术和分化鉴定其真实性。在妊娠第14天,麻醉家兔,将hWJ-MSCs注射到24个胎儿的子宫内。22个胎儿成功出生。采用注射胚胎制备兔肝标本10例,包括;出生后第3天8只,出生后第21天2只。未注射的胎儿被认为是阳性对照。将对照组和hwj - mscs处理的家兔的肝脏固定、处理、染色,并通过体视学方法进行检查。结果:与对照组相比,hwj - mscs治疗组肝脏平均重量和体积分别增加42%和78%。肝细胞体积增加63%,肝窦体积增加3倍。中心静脉的总容积增加了70%。与对照组相比,实验组肝细胞总数增加了112%。实验组肝细胞核总体积较对照组增大117%。结论:人间充质干细胞异种移植后,宿主组织微环境(这里是兔肝脏)发生了改变,其中包括肝组织和肝细胞形态计量指标对应的定量因素。
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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.
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