Oral microbiome diversity: The curious case of Corynebacterium sp. isolation.

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2022-10-01 Epub Date: 2022-08-01 DOI:10.1111/omi.12381
Puthayalai Treerat, Brian McGuire, Elizabeth Palmer, Erin M Dahl, Lisa Karstens, Justin Merritt, Jens Kreth
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引用次数: 2

Abstract

Oral microbiome sequencing efforts revealed the presence of hundreds of different microbes. Interindividual differences at strain and species resolution suggest that microbiome diversity could lead to mechanistically distinct gene regulation as well as species-related differences in phenotypes. Commonly, gene regulation and related phenotypes are studied in a few selected strains of a particular species with conclusions that are mostly generalized. The aim of this study was to isolate several species of Corynebacterium using an established protocol that led to the previous isolation of C. durum. Characterization of C. durum interspecies interactions revealed a specific mechanism for chain elongation in Streptococcus sanguinis that was the result of corynebacterial fatty acid production and secretion. While the protocol was successfully applied to isolate what we presumed to be additional Corynebacterium based on several phenotypic traits that seem to be identical to C. durum, genome sequencing of the newly isolated strains placed them closer to Actinomyces. Both Corynebacterium and Actinomyces are suborders of the Actinobacteridae and related species. Our study suggests to take several comprehensive strategies into consideration when taxonomically identifying closely related microorganisms. Furthermore, it seems to be important to test common core phenotypes in bacterial ecology to understand the behavior of specific groups of microbes, rather than simply relying upon genome sequence homology to establish relationships in the microbiome.

口腔微生物组多样性:棒状杆菌分离的奇怪案例。
口腔微生物组测序工作揭示了数百种不同微生物的存在。菌株和物种分辨率的个体间差异表明,微生物组多样性可能导致机制上不同的基因调节以及表型上与物种相关的差异。通常,在特定物种的少数选定菌株中研究基因调控和相关表型,其结论大多是一般性的。这项研究的目的是使用一种既定的方案分离出几种棒状杆菌,该方案导致了之前分离出的硬脊杆菌。硬链菌种间相互作用的特征揭示了血链球菌链伸长的特定机制,这是棒状杆菌脂肪酸产生和分泌的结果。虽然该方案成功地应用于分离出了我们认为是额外的棒状杆菌,基于几个似乎与硬硬链菌相同的表型特征,但新分离菌株的基因组测序使它们更接近放线菌。棒状杆菌和放线菌都是放线菌科的亚目和相关物种。我们的研究建议,在分类鉴定密切相关的微生物时,应考虑几种综合策略。此外,测试细菌生态学中的常见核心表型以了解特定微生物群的行为似乎很重要,而不是简单地依靠基因组序列同源性来建立微生物组中的关系。
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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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