Establishment and inheritance of minichromosomes from Arabidopsis haploid induction.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Ek Han Tan, Benny Ordoñez, Tejas Thondehaalmath, Danelle K Seymour, Julin N Maloof, Ravi Maruthachalam, Luca Comai
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引用次数: 1

Abstract

Minichromosomes are small, sometimes circular, rearranged chromosomes consisting of one centromere and short chromosomal arms formed by treatments that break DNA, including plant transformation. Minichromosomes have the potential to serve as vectors to quickly move valuable genes across a wide range of germplasm, including into adapted crop varieties. To realize this potential, minichromosomes must be reliably generated, easily manipulated, and stably inherited. Here we show a reliable method for minichromosome formation in haploids resulting from CENH3-mediated genome elimination, a process that generates genome instability and karyotypic novelty specifically on one parental genome. First, we identified 2 out of 260 haploids, each containing a single-copy minichromosome originating from centromeric regions of chromosomes 1 and 3, respectively. The chromosome 1 minichromosome we characterized did not pair at meiosis but displayed consistent transmission over nine selfing generations. Next, we demonstrated that CENH3-based haploid induction can produce minichromosomes in a targeted manner. Haploid inducers carrying a selectable pericentromeric marker were used to isolate additional chromosome-specific minichromosomes, which occurred in 3 out of 163 haploids. Our findings document the formation of heritable, rearranged chromosomes, and we provide a method for convenient minichromosome production.

Abstract Image

Abstract Image

Abstract Image

拟南芥单倍体诱导小染色体的建立与遗传。
小染色体是小的,有时是圆形的,由一个着丝粒和短染色体臂组成的重排染色体,由DNA断裂处理形成,包括植物转化。小染色体有潜力作为载体,在广泛的种质资源中快速移动有价值的基因,包括进入适应的作物品种。为了实现这一潜力,必须可靠地产生小染色体,易于操作,并稳定地遗传。在这里,我们展示了由cenh3介导的基因组消除导致的单倍体小染色体形成的可靠方法,这一过程产生基因组不稳定和核型新颖性,特别是在一个亲本基因组上。首先,我们从260个单倍体中鉴定出2个单倍体,每个单倍体分别含有来自染色体1和3的着丝粒区域的单拷贝小染色体。我们所鉴定的1号染色体小染色体在减数分裂时没有配对,但在9个自交代中表现出一致的遗传。接下来,我们证明了基于cenh3的单倍体诱导可以有针对性地产生小染色体。采用单倍体诱导剂携带一个可选择的周中心点标记,分离出额外的染色体特异性小染色体,163个单倍体中有3个出现了小染色体。我们的发现记录了可遗传的、重排的染色体的形成,我们提供了一种方便的小染色体生产方法。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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