Diagnostic utility of C4d immunohistochemistry in membranous nephropathy.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01

Bheemanathi Hanuman Srinivas, Norton Stephen, Priyamvada Ps

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引用次数: 0

Abstract

Objectives: Membranous nephropathy (MN), also called membranous glomerulopathy, is one of the leading causes of nephrotic syndrome in adults which is defined by the presence of subepithelial immune complex deposits with a spectrum of changes in the glomerular basement membrane (GBM). It is known that C4d is a byproduct of the classic and lectin pathway. There is deposition of C4d noted in the cases of immune complex-mediated glomerulonephritis involving the classical/lectin pathway including MN. The main objective of this study is to assess the utility C4d as an immunohistochemical (IHC) stain in MN.

Materials: A total of 43 cases of MN (primary & secondary) were taken, and 39 cases of minimal change disease (MCD)/focal segmental glomerulosclerosis (FSGS) were used as the control group. All the relevant data were retrieved from the hospital database. C4d immunohistochemistry was performed in the cases as well as the control group.

Results: A diffuse continuous staining pattern in the glomeruli was observed in cases of primary MN whereas a discontinuous staining in the glomerulI favors a secondary MN. 26/29 cases of MCD showed positivity in the podocytes. Among the cases of FSGS, 7/10 cases showed positivity in the podocytes with 3 cases showing an associated mesangial blush pattern of staining.

Conclusion: Very few studies are available demonstrating the importance of C4d IHC in MN. C4d IHC can be a useful adjunct for immunofluorescence, especially in cases of early MN.

本刊更多论文

C4d免疫组织化学在膜性肾病中的诊断价值。

目的:膜性肾病(MN)，也称为膜性肾小球病，是成人肾病综合征的主要原因之一，其定义为肾小球基底膜(GBM)中存在上皮下免疫复合物沉积，并伴有一系列变化。众所周知，C4d是经典和凝集素途径的副产品。免疫复合物介导的肾小球肾炎涉及经典/凝集素途径(包括MN)，可见C4d沉积。本研究的主要目的是评估C4d作为免疫组化(IHC)染色在MN中的应用。材料:选取原发性和继发性MN 43例，以最小改变病(MCD)/局灶节段性肾小球硬化(FSGS) 39例为对照组。所有相关数据均从医院数据库中检索。病例及对照组均行C4d免疫组化。结果:原发性肾小球呈弥漫性连续染色，继发性肾小球呈不连续染色。26/29例MCD足细胞呈阳性。在FSGS病例中，7/10的足细胞呈阳性，其中3例伴系膜红色染色。结论:很少有研究表明C4d IHC在MN中的重要性。C4d免疫组化可以作为一种有用的免疫荧光辅助手段，特别是在早期MN病例中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.