Response Surface Optimization for Investigating Antioxidant Potential of Camellia Sinensis and Withania Somnifera in Synergistic Manner.

Q2 Agricultural and Biological Sciences

Proceedings of the National Academy of Sciences, India. Section B Pub Date : 2023-01-01 Epub Date: 2022-11-01 DOI:10.1007/s40011-022-01423-6

Arti Devi, Vagish Dwibedi, Nancy George, Zaved Ahmed Khan

{"title":"Response Surface Optimization for Investigating Antioxidant Potential of Camellia Sinensis and Withania Somnifera in Synergistic Manner.","authors":"Arti Devi, Vagish Dwibedi, Nancy George, Zaved Ahmed Khan","doi":"10.1007/s40011-022-01423-6","DOIUrl":null,"url":null,"abstract":"The paper highlights the synergistic potential of the novel combination of Camellia sinensis (Kangra green tea) and Withania somnifera (Ashwagandha). One variable at a time approach was used to find antioxidant potential of C. sinensis and W. somnifera alone and in combination. Optimization of antioxidant potential was done by using different plant concentrations of C. sinensis and W. somnifera using a statistical approach of central composite design (CCD) of RSM (response surface methodology). Initial antioxidant activity during optimization of the solvent system was observed in methanol for C. sinensis with DPPH, superoxide radical scavenging assay and hydrogen peroxide scavenging assay (44.9 ± 0.62, 43.77 ± 0.10, 43.88 ± 0.10% scavenging) and for W. somnifera (40.22 ± 0.39, 43.29 ± 1.12, 41.88 ± 0.11% scavenging), respectively. Initially, IC50 has been calculated for C. sinensis (235.26 ± 0.012 μg/mL) and W. somnifera (256.39 ± 0.43 μg/mL) in methanol. Before statistical optimization, the maximum synergistic antioxidant potential of C. sinensis (200 μg/mL) and W. somnifera (150 μg/mL) with DPPH assay, superoxide radical scavenging and hydrogen peroxide scavenging assay was found to be 56.57 ± 0.62, 56.99 ± 0.42, 55.44 ± 0.53% scavenging, respectively. IC50 value has been calculated for C. sinensis + W. somnifera (IC50 = 215.47 ± 0.06 μg/mL). Optimization of plant concentration using CCD of RSM resulted in enhancement of antioxidant potential of C. sinensis (200.5 μg/mL) and W. somnifera (200.5 μg/mL) was found to be 78.01 ± 0.01% scavenging when compared to the initial antioxidant potential, i.e., 56.57 ± 0.62% scavenging shows a 1.37-fold increase from initial antioxidant potential. Research unveils that using various combination of C. sinensis and W. somnifera enhance the antioxidant potential in vitro.Supplementary information: The online version contains supplementary material available at 10.1007/s40011-022-01423-6.","PeriodicalId":54564,"journal":{"name":"Proceedings of the National Academy of Sciences, India. Section B","volume":"93 2","pages":"397-408"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628569/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the National Academy of Sciences, India. Section B","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s40011-022-01423-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/11/1 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

The paper highlights the synergistic potential of the novel combination of Camellia sinensis (Kangra green tea) and Withania somnifera (Ashwagandha). One variable at a time approach was used to find antioxidant potential of C. sinensis and W. somnifera alone and in combination. Optimization of antioxidant potential was done by using different plant concentrations of C. sinensis and W. somnifera using a statistical approach of central composite design (CCD) of RSM (response surface methodology). Initial antioxidant activity during optimization of the solvent system was observed in methanol for C. sinensis with DPPH, superoxide radical scavenging assay and hydrogen peroxide scavenging assay (44.9 ± 0.62, 43.77 ± 0.10, 43.88 ± 0.10% scavenging) and for W. somnifera (40.22 ± 0.39, 43.29 ± 1.12, 41.88 ± 0.11% scavenging), respectively. Initially, IC₅₀ has been calculated for C. sinensis (235.26 ± 0.012 μg/mL) and W. somnifera (256.39 ± 0.43 μg/mL) in methanol. Before statistical optimization, the maximum synergistic antioxidant potential of C. sinensis (200 μg/mL) and W. somnifera (150 μg/mL) with DPPH assay, superoxide radical scavenging and hydrogen peroxide scavenging assay was found to be 56.57 ± 0.62, 56.99 ± 0.42, 55.44 ± 0.53% scavenging, respectively. IC₅₀ value has been calculated for C. sinensis + W. somnifera (IC₅₀ = 215.47 ± 0.06 μg/mL). Optimization of plant concentration using CCD of RSM resulted in enhancement of antioxidant potential of C. sinensis (200.5 μg/mL) and W. somnifera (200.5 μg/mL) was found to be 78.01 ± 0.01% scavenging when compared to the initial antioxidant potential, i.e., 56.57 ± 0.62% scavenging shows a 1.37-fold increase from initial antioxidant potential. Research unveils that using various combination of C. sinensis and W. somnifera enhance the antioxidant potential in vitro.

Supplementary information: The online version contains supplementary material available at 10.1007/s40011-022-01423-6.

Abstract Image

查看原文本刊更多论文

响应面优化法研究山茶花和紫薇协同抗氧化潜力。

该论文强调了中华山茶（Kangra绿茶）和紫薇（Ashwagandha）的新组合的协同潜力。采用一次一个变量的方法来研究中华鳖和桑蚕单独和联合的抗氧化潜力。采用响应面法（RSM）的中心复合设计（CCD）统计方法，利用不同植物浓度的中华绒螯蟹和桑蚕对其抗氧化能力进行了优化。用DPPH、超氧化物自由基清除试验和过氧化氢清除试验在甲醇中观察了溶剂体系优化过程中中华鳖的初始抗氧化活性（44.9 ± 0.62，43.77 ± 0.10，43.88 ± 0.10%的清除率）和对W.somnifera（40.22 ± 0.39、43.29 ± 1.12，41.88 ± 0.11%清除）。最初，已经计算出中华鳖的IC50（235.26 ± 0.012μg/mL）和W.somnifera（256.39 ± 0.43μ。在统计优化之前，中华绒螯蟹（200μg/mL）和桑蚕（150μg/mL）在DPPH测定、超氧化物自由基清除和过氧化氢清除测定中的最大协同抗氧化潜力为56.57 ± 0.62，56.99 ± 0.42，55.44 ± 清除率分别为0.53%。计算了中华鳖的IC50值 + W.somnifera（IC50 = 215.47 ± 0.06μg/mL）。利用RSM的CCD优化植物浓度可提高中华绒螯蟹（200.5μg/mL ± 与初始抗氧化潜力相比，清除率为0.01%，即56.57 ± 0.62%的清除率显示出比初始抗氧化潜力增加1.37倍。研究表明，使用C。sinensis和W。somnifera能增强体外抗氧化能力。补充信息：在线版本包含补充材料，可访问10.1007/s40011-022-01423-6。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Proceedings of the National Academy of Sciences, India. Section B BIOLOGY-

CiteScore

3.20

自引率

0.00%

发文量

133

审稿时长

6-12 weeks

期刊介绍： The Proceedings of the National Academy of Sciences, India, Section B: Biological Sciences, is one of the oldest journals of India, launched in the year 1930, by the National Academy of Sciences, India (the Oldest Science Academy of India). The research/review papers of different fields of science, e.g. Agriculture Sciences (Agriculture, Animal Husbandry, Fisheries, Forestry, Agric. Toxicology, Soil Science, Plant Protection, Post Harvest Technology, and Agricultural Engineering), Animal Sciences (Structural, Developmental, Functional, Genetical, Ecological, Behavioural, Taxonomical and Evolutionary Aspects), Biochemistry, Biophysics, Biotechnology (including Molecular and Cell Biology, Structural and Functional Studies, Microbiology and Immunology), Medical & Forensic Sciences (Basic and Clinical Medical Sciences, Pharmacology, Anthropology, Psychology and Forensic Sciences, Human genetics, Reproduction Biology, Neurosciences and Molecular Medicine) and Plant Sciences (Structural, Developmental, Functional, Genetical, Ecological, Taxonomical and Evolutionary Aspects), are published in this journal for dissemination of the scientific knowledge and research. The papers published are indexed/abstracted by the leading abstracting agencies of the world. The papers are published after critical review and editing by the eminent experts of the concerned subject area; therefore, the quality publication is assured once the paper is accepted by the learned referees.