A Clinical Scoring Systems for the Evaluation of Corynebacterium bovis -associated Disease in NSG Mice.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Christopher A Manuel, Emily C Pearson, Umarani Pugazhenthi, Michael K Fink, Lauren M Habenicht, Derek L Fong, Jori K Leszczynski, Michael J Schurr
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引用次数: 0

Abstract

Clinical signs of Corynebacterium bovis infections are well-known in athymic nude mice. However, C. bovis can also infect and cause clinical signs in many hirsute, immunocompromised mouse strains such as NSG (NOD. Cg-Prkdcscid Il2rgtm1Wgl/SzJ). Typically, the clinical assessment of C. bovis-infected mice begins when overt clinical signs are initially observed and thus the early course of infection has not been thoroughly described. The goal of this study was to characterize the clinical progression of C. bovis infection in NSG mice under experimental conditions and develop a quantifiable clinical scoring system. For the development and application of this clinical scoring system, 54 naïve NSG mice were exposed to soiled bedding from clinically ill C. bovis-infected NSG mice and the emergence of clinical signs was monitored and scored weekly for 8 wk. Overall, we identified 6 benchmark changes associated with C. bovis clinical infection. Four changes were the appearance of the eyes, ears, hair coat, and posture. Two behavioral changes were increased grooming activity and rapid head shaking. All clinical signs appeared consistently and progressed temporally with increasing clinical severity. Characterization of clinical signs and scoring of clinical disease will aid veterinarians in the assessment of C. bovis-infected NSG mice and may help in the evaluation of current and future clinical interventions used to prevent or treat C. bovis-infected immunodeficient mice.

NSG小鼠牛棒状杆菌相关疾病的临床评分系统
牛棒状杆菌感染的临床症状在胸腺裸鼠中是众所周知的。然而,C. bovis也可以感染许多多毛、免疫功能低下的小鼠品系,如NSG (NOD),并引起临床症状。Cg-Prkdcscid Il2rgtm1Wgl / SzJ)。通常,当最初观察到明显的临床症状时,对牛链球菌感染小鼠的临床评估就开始了,因此感染的早期过程尚未得到彻底的描述。本研究的目的是表征实验条件下NSG小鼠C. bovis感染的临床进展,并建立可量化的临床评分系统。为了开发和应用该临床评分系统,将54只naïve NSG小鼠暴露于临床上感染牛曲霉的NSG小鼠的脏被褥中,每周监测临床症状的出现并评分,持续8周。总的来说,我们确定了6个与牛C.临床感染相关的基准变化。四个变化是眼睛、耳朵、毛发和姿势的外观。两项行为变化是梳洗活动增加和快速摇头。所有临床症状表现一致,并随临床严重程度的增加而有时间进展。临床症状的表征和临床疾病的评分将有助于兽医评估牛C.感染的NSG小鼠,并可能有助于评估当前和未来用于预防或治疗牛C.感染的免疫缺陷小鼠的临床干预措施。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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