Soft-tissue infection due to Mycoplasma hominis.

Abstract

Gram staining of the right hand dorsum collection showed numerous polymorphonuclear leukocytes and no visible microorganisms. However, 4 days of incubation on blood agar at 35°C under 5% CO2 resulted in the formation of pinpoint-sized colonies resembling water droplets. These colonies could not be identified by matrix-assisted laser desorption ionization– time of flight mass spectrometry (MALDI-TOF MS) (“no peaks found”). Gram and Ziehl–Nielsen stainings performed on the colonies showed no bacteria. These results led us to suspect Mycoplasma spp, and the culture were transferred to Reference Laboratory for ARNr16s PCR and subsequent sequencing, where this microorganism was detected and identified.