Keratinocyte Proliferation and Hemidesmosome Formation on Surfaces for Dental Implants: In Vitro Study.

IF 1.7 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Jordi Gargallo-Albiol, Nicholas G Fischer, Conrado Aparicio, Hom-Lay Wang
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引用次数: 0

Abstract

Purpose: To demonstrate the likelihood of the polyetheretherketone (PEEK), zirconia (ZrO2), and titanium (Ti) disks to support proliferation and hemidesmosome formation of gingival cells.

Materials and methods: Water contact angle was performed on each material, and surface roughness (Ra) was measured. Scanning electron microscopy and x-ray photoelectron spectroscopy were used. Later, oral keratinocyte cells were cultured on disks, and metabolic activity and expression of hemidesmosome markers, integrin α6 and β4, in relation to the biomaterial disks at 1, 3, and 5 days of cell culture were quantified. Tissue culture polystyrene was used as the control. Statistical analysis was performed with analysis of variance (ANOVA) with Tukey post hoc comparison test. A P value of < .05 was considered statistically significant.

Results: The water contact angle ranged from 70.2 degrees (Ti) to a maximum of hydrophobicity of 93.3 degrees (PEEK). Ra was highest on ZrO2, followed by PEEK. Ti showed the most keratinocyte metabolic activity at 1, 3, and 5 culture periods. Contrarily, ZrO2 and PEEK disks had lower keratinocyte metabolic activity at all observation times, with no statistical differences between both groups. Integrin α6 and β4 expression was highest on TCPS and ZrO2 compared to Ti and PEEK.

Conclusion: Keratinocytes proliferated faster on Ti than on ZrO2 and PEEK substrates, and expression of hemidesmosome formation markers, integrin α6 and β4, were higher on ZrO2 than either Ti or PEEK. Int J Oral Maxillofac Implants 2023;38:496-502. doi: 10.11607/jomi.9894.

牙种植体表面角质细胞增殖和半粒体形成:体外研究。
目的:证明聚醚醚酮(PEEK)、氧化锆(ZrO2)和钛(Ti)圆盘支持牙龈细胞增殖和半粒体形成的可能性。材料和方法:对每种材料进行水接触角测量,并测量表面粗糙度Ra。采用扫描电镜和x射线光电子能谱分析。随后,将口腔角质形成细胞在圆盘上培养,定量细胞培养1、3、5 d时半粒酶标记物整合素α6、β4与生物材料圆盘的代谢活性和表达。以组织培养聚苯乙烯为对照。统计学分析采用方差分析(ANOVA)和Tukey事后比较检验。P值< 0.05认为有统计学意义。结果:水接触角范围从70.2°(Ti)到最大疏水性93.3°(PEEK)。ZrO2上Ra含量最高,PEEK次之。Ti在第1、3、5个培养周期的角质细胞代谢活性最高。相反,在所有观察时间,ZrO2和PEEK磁盘的角质细胞代谢活性较低,两组之间无统计学差异。与Ti和PEEK相比,整合素α6和β4在TCPS和ZrO2中的表达最高。结论:角质形成细胞在Ti基质上的增殖速度快于ZrO2和PEEK基质,且半胱氨酸形成标志物整合素α6和β4在ZrO2基质上的表达高于Ti和PEEK基质。口腔颌面种植[J]; 2009;38(8):496- 496。doi: 10.11607 / jomi.9894。
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来源期刊
CiteScore
3.30
自引率
5.00%
发文量
115
审稿时长
6 months
期刊介绍: Edited by Steven E. Eckert, DDS, MS ISSN (Print): 0882-2786 ISSN (Online): 1942-4434 This highly regarded, often-cited journal integrates clinical and scientific data to improve methods and results of oral and maxillofacial implant therapy. It presents pioneering research, technology, clinical applications, reviews of the literature, seminal studies, emerging technology, position papers, and consensus studies, as well as the many clinical and therapeutic innovations that ensue as a result of these efforts. The editorial board is composed of recognized opinion leaders in their respective areas of expertise and reflects the international reach of the journal. Under their leadership, JOMI maintains its strong scientific integrity while expanding its influence within the field of implant dentistry. JOMI’s popular regular feature "Thematic Abstract Review" presents a review of abstracts of recently published articles on a specific topical area of interest each issue.
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