Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus.

IF 0.9 4区 生物学
George A Penman, Deborah E A Lockhart, Andrew Ferenbach, Daan M F van Aalten
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引用次数: 4

Abstract

Aspergillus fumigatus UDP-galactopyranose mutase (AfUGM) is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen. AfUGM was recombinantly produced in Escherichia coli, purified and crystallized by the sitting-drop method, producing orthorhombic crystals that diffracted to a resolution of 3.25 Å. The crystals contained four molecules per asymmetric unit and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 127.72, b = 134.30, c = 173.84 Å. Incorporation of selenomethionine was achieved, but the resulting crystals did not allow solution of the phase problem.

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烟曲霉udp -半乳糖酰脲酶的纯化、结晶及x射线衍射初步数据。
烟曲霉UDP吡喃半乳糖突变酶(AfUGM)是一种潜在的药物靶点,参与这种真菌病原体细胞壁的合成。AfUGM在大肠杆菌中重组产生,通过坐滴法纯化和结晶,产生衍射至3.25Å分辨率的正交晶体。晶体每个不对称单元包含四个分子,属于空间群P2(1)2(1)1(1),晶胞参数a=127.72,b=134.30,c=173.84Å。硒代蛋氨酸的掺入得以实现,但所得晶体不允许相问题的解决。
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来源期刊
自引率
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审稿时长
2-4 weeks
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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