Sphingosine-1-phosphate regulation of luteinising hormone-induced steroidogenesis and proliferation of bovine theca cells in vitro.

IF 1.8 4区 生物学 Q3 DEVELOPMENTAL BIOLOGY
Zaire B Medina-Moctezuma, Cyndi G Hernández-Coronado, Lydia Marín-López, Adrián Guzmán, David González-Aretia, Carlos G Gutiérrez, Ana Ma Rosales-Torres
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引用次数: 1

Abstract

Context: Sphingosine-1-phosphate (S1P) is synthesised by follicle granulosa cells under the influence of follicle-stimulating hormone and seems to be necessary for the biological effects of this gonadotrophin.

Aims: To determine if luteinising hormone (LH) increases S1P production and if this sphingolipid, either induced by LH or added to culture media, regulates steroidogenesis and cell viability in bovine theca cells.

Methods: We used bovine theca cell cultures treated with: S1P (0, 0.1, 1 and 10μM; Experiment 1), LH (0, 0.02, 0.2 and 2ngmL-1 ; Experiment 2) and LH (0.02ngmL-1 ) plus a sphingosine kinase inhibitor (SKI-178; 0, 5 and 10μM; Experiment 3).

Key results: Treatment with S1P did not affect (P >0.05) theca cell viability or their ability to produce progesterone and testosterone. LH (0.02ngmL-1 ) increased (P <0.05) S1P production, and stimulated the expression of phosphorylated sphingosine kinase-1 (pSPHK1). However, the inhibition of SPHK1, by a specific SPHK1 inhibitor (SKI-178), reduced (P <0.05) cell viability and progesterone secretion. Additionally, the use of SKI-178 increased theca cell testosterone production (P<0.05).

Conclusions: S1P added to culture media did not affect cell viability or steroid synthesis. However, LH stimulated the production of S1P, by increasing phosphorylation of SPHK1 in theca cells. This intracellular S1P was inhibitory on testosterone production but augmented progesterone and viable cell number.

Implications: These results suggest a novel signalling pathway for LH in theca cells and underline the importance of S1P in the regulation of steroid synthesis.

鞘氨醇-1-磷酸对黄体生成素诱导的牛卵泡膜细胞体外类固醇生成和增殖的调控。
背景:鞘氨醇-1-磷酸(S1P)是由卵泡颗粒细胞在促卵泡激素的作用下合成的,似乎是这种促性腺激素的生物学效应所必需的。目的:确定黄体生成素(LH)是否能增加S1P的产生,以及这种鞘脂,无论是由LH诱导还是添加到培养基中,是否能调节牛卵泡膜细胞的类固醇生成和细胞活力。方法:采用S1P(0、0.1、1、10μM)处理的牛卵泡膜细胞培养;实验1),LH(0、0.02、0.2、2ngmL-1;实验2)和LH (0.02ngmL-1)加鞘氨醇激酶抑制剂(SKI-178;0、5、10μM;实验3).关键结果:S1P对卵泡膜细胞活力及孕酮、睾酮分泌能力无显著影响(P >0.05)。LH (0.02ngmL-1)升高(P)。结论:培养基中添加S1P不影响细胞活力和类固醇合成。然而,LH通过增加卵泡细胞中SPHK1的磷酸化来刺激S1P的产生。胞内S1P抑制睾酮的产生,但增加孕酮和活细胞数。意义:这些结果提示了卵泡膜细胞中LH的新信号通路,并强调了S1P在类固醇合成调节中的重要性。
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来源期刊
CiteScore
2.10
自引率
10.50%
发文量
317
审稿时长
2 months
期刊介绍: Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management. Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students. Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology. Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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