Urinary shedding of common DNA viruses and their possible association with bladder cancer: a qPCR-based study.

IF 2 4区 医学 Q3 ONCOLOGY
Jan Hrbáček, Vítězslav Hanáček, Dominika Kadlečková, Adéla Cirbusová, Pavel Čermák, Ruth Tachezy, Roman Zachoval, Martina Saláková
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引用次数: 1

Abstract

After a decade of human urinary microbiota research, little is known about the composition of the urinary virome and its association with health and disease. This study aimed to investigate the presence of 10 common DNA viruses in human urine and their putative association with bladder cancer (BC). Catheterized urine samples were collected from patients undergoing endoscopic urological procedures under anesthesia. After DNA extraction from the samples, viral DNA sequences were detected using real-time PCR. Viruria rates were compared between BC patients and controls. A total of 106 patients (89 males and 17 females) were included in the study. Fifty-seven (53.8%) were BC patients and 49 (46.2%) had upper urinary tract stones or bladder outlet obstruction. The viruses detected in the urine were human cytomegalovirus (2.0%), Epstein-Barr virus (6.0%), human herpesvirus-6 (12.5%), human papillomavirus (15.2%), BK polyomavirus (15.5%), torque teno virus (44.2%), and JC polyomavirus (47.6%), while no adenoviruses, herpes simplex virus 1 and 2, or parvoviruses were found. There were statistically significant differences in HPV viruria rates between cancer patients and controls (24.5% vs. 4.3%, p=0.032 after adjustment for age and gender). Viruria rates increased from benign to non-muscle-invasive and muscle-invasive tumors. Patients with a history of BC have higher HPV viruria rates than controls. Whether this relationship is a causal one remains to be established by further research.

尿中常见DNA病毒的脱落及其与膀胱癌的可能关联:一项基于qpcr的研究
经过十年的人类泌尿微生物群研究,人们对泌尿病毒组的组成及其与健康和疾病的关系知之甚少。本研究旨在探讨人类尿液中10种常见DNA病毒的存在及其与膀胱癌(BC)的可能关系。在麻醉下接受内镜泌尿外科手术的患者收集导管尿样本。从样品中提取DNA后,采用实时荧光定量PCR检测病毒DNA序列。比较BC患者和对照组的病毒率。106例患者(男性89例,女性17例)纳入研究。57例(53.8%)为BC患者,49例(46.2%)为上尿路结石或膀胱出口梗阻。尿中检出的病毒为人巨细胞病毒(2.0%)、eb病毒(6.0%)、人疱疹病毒-6(12.5%)、人乳头瘤病毒(15.2%)、BK多瘤病毒(15.5%)、torque teno病毒(44.2%)、JC多瘤病毒(47.6%),未检出腺病毒、单纯疱疹病毒1、单纯疱疹病毒2和细小病毒。肿瘤患者与对照组HPV病毒感染率差异有统计学意义(24.5% vs. 4.3%,调整年龄和性别后p=0.032)。从良性肿瘤到非肌肉侵袭性和肌肉侵袭性肿瘤,病毒率均有所增加。有BC病史的患者HPV病毒感染率高于对照组。这种关系是否存在因果关系还有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Neoplasma
Neoplasma 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
238
审稿时长
3 months
期刊介绍: The journal Neoplasma publishes articles on experimental and clinical oncology and cancer epidemiology.
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