Identification and validation of biomarkers related to Th1 cell infiltration in neuropathic pain.

IF 4.4 3区医学 Q2 IMMUNOLOGY

Journal of Inflammation-London Pub Date : 2023-06-01 DOI:10.1186/s12950-023-00343-0

Xiangsheng Zhang, Jiurong Cheng, Yingdong Deng, Caiyun Guo, Yu Cao, Suo Wang, Chenxi Zhou, Ziqiang Lin, Simin Tang, Jun Zhou

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引用次数: 1

Abstract

Neuropathic pain (NP) is a widespread chronic pain with a prevalence of 6.9-10% in the general population, severely affecting patients' physical and mental health. Accumulating evidence indicated that the immune environment is an essential factor causing NP. However, the mechanism is unclear. This study attempted to analyze NP-related immune infiltration patterns. We downloaded the expression profiles from the Gene Expression Omnibus (GEO) database. The novel method of single-sample gene set enrichment analysis (ssGSEA) algorithm and weighted gene co-expression network analysis (WGCNA) was applied to identify immune-related genes and verified in vitro and in vivo experiments. The spared nerve injury (SNI) group was closely related to type1 T helper cells (Th1 cells), and two key genes (Abca1 and Fyb) positively correlated with Th1 cell infiltration. At the single-cell level, Abca1 and Fyb were significantly expressed in macrophages. In addition, we verified that Abca1 could affect the function of macrophages. Finally, we hypothesized that Abca1 is involved in the infiltration of Th1 cells into dorsal root ganglion (DRG) tissues and induces NP via immunoinflammatory response. Hence, the present study aimed to elucidate the correlation between NP and neuroinflammation and identify a new therapeutic target for treating NP.

Abstract Image

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神经性疼痛中Th1细胞浸润相关生物标志物的鉴定与验证。

神经性疼痛(Neuropathic pain, NP)是一种普遍存在的慢性疼痛，在普通人群中患病率为6.9-10%，严重影响患者的身心健康。越来越多的证据表明，免疫环境是引起NP的重要因素。然而，其机制尚不清楚。本研究试图分析np相关的免疫浸润模式。我们从Gene expression Omnibus (GEO)数据库中下载了表达谱。应用单样本基因集富集分析(ssGSEA)算法和加权基因共表达网络分析(WGCNA)的新方法鉴定免疫相关基因，并在体外和体内实验中进行验证。SNI组与1型T辅助细胞(Th1细胞)密切相关，两个关键基因(Abca1和Fyb)与Th1细胞浸润呈正相关。在单细胞水平上，Abca1和Fyb在巨噬细胞中显著表达。此外，我们证实Abca1可以影响巨噬细胞的功能。最后，我们假设Abca1参与Th1细胞向背根神经节(DRG)组织的浸润，并通过免疫炎症反应诱导NP。因此，本研究旨在阐明NP与神经炎症的关系，并寻找治疗NP的新靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation-London IMMUNOLOGY-

CiteScore

7.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Inflammation welcomes research submissions on all aspects of inflammation. The five classical symptoms of inflammation, namely redness (rubor), swelling (tumour), heat (calor), pain (dolor) and loss of function (functio laesa), are only part of the story. The term inflammation is taken to include the full range of underlying cellular and molecular mechanisms involved, not only in the production of the inflammatory responses but, more importantly in clinical terms, in the healing process as well. Thus the journal covers molecular, cellular, animal and clinical studies, and related aspects of pharmacology, such as anti-inflammatory drug development, trials and therapeutic developments. It also considers publication of negative findings. Journal of Inflammation aims to become the leading online journal on inflammation and, as online journals replace printed ones over the next decade, the main open access inflammation journal. Open access guarantees a larger audience, and thus impact, than any restricted access equivalent, and increasingly so, as the escalating costs of printed journals puts them outside University budgets. The unrestricted access to research findings in inflammation aids in promoting dynamic and productive dialogue between industrial and academic members of the inflammation research community, which plays such an important part in the development of future generations of anti-inflammatory therapies.