MiR-30e-5p deficiency exerts an inhibitory effect on inflammation in rheumatoid arthritis via regulating Atl2 expression.

IF 1.1 4区医学 Q4 Medicine

Archives of rheumatology Pub Date : 2023-03-01 DOI:10.46497/ArchRheumatol.2023.9526

Shanshan Liu, Kai Wang, Ju Li, Yan Liu, Zhongyuan Zhang, Deqian Meng

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引用次数: 1

Abstract

Objectives: This study aims to investigate the inflammatory effect of the microRNA (miRNA) miR-30e-5p on rheumatoid arthritis (RA) development in RA mice and fibroblast-like synoviocytes (FLS).

Materials and methods: MiR-30e-5p and atlastin GTPase 2 (Atl2) expression in RA tissues and RA-FLS was evaluated using real-time quantitative polymerase chain reaction. The function of miR-30e-5p in inflammation of RA mice and RA-FLS was analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. 5-ethynyl-2'-deoxyuridine (EdU) assay was used to detect RA-FLS proliferation. Luciferase reporter assay was to confirm the interaction between miR-30e-5p and Atl2.

Results: MiR-30e-5p expression was upregulated in the tissues from RA mice. Silencing miR-30e-5p alleviated inflammation in RA mice and RA-FLS. MiR-30e-5p negatively modulated Atl2 expression. Atl2 knockdown exerted a proinflammatory effect on RA-FLS. Atl2 knockdown rescued the inhibitory effect of miR-30e-5p knockdown on proliferation and inflammatory response of RA-FLS.

Conclusion: MiR-30e-5p knockdown inhibited the inflammatory response in RA mice and RA-FLS through Atl2.

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MiR-30e-5p缺乏通过调节Atl2的表达对类风湿关节炎炎症产生抑制作用。

目的:本研究旨在探讨microRNA (miRNA) miR-30e-5p对类风湿关节炎(RA)小鼠和成纤维细胞样滑膜细胞(FLS)发展的炎症作用。材料和方法:采用实时定量聚合酶链反应检测MiR-30e-5p和atlastin GTPase 2 (Atl2)在RA组织和RA- fls中的表达。采用酶联免疫吸附试验(ELISA)和Western blotting分析miR-30e-5p在RA小鼠炎症和RA- fls中的功能。采用5-乙基-2'-脱氧尿苷(EdU)法检测RA-FLS的增殖。荧光素酶报告试验证实miR-30e-5p与Atl2之间的相互作用。结果:MiR-30e-5p在RA小鼠组织中表达上调。沉默miR-30e-5p可减轻RA小鼠和RA- fls的炎症。MiR-30e-5p负向调节Atl2的表达。Atl2敲低对RA-FLS有促炎作用。敲除Atl2后，miR-30e-5p敲除对RA-FLS增殖和炎症反应的抑制作用得以恢复。结论:MiR-30e-5p敲低可通过Atl2抑制RA小鼠和RA- fls的炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of rheumatology Medicine-Rheumatology

CiteScore

2.00

自引率

9.10%

发文量

期刊介绍： The Archives of Rheumatology is an official journal of the Turkish League Against Rheumatism (TLAR) and is published quarterly in March, June, September, and December. It publishes original work on all aspects of rheumatology and disorders of the musculoskeletal system. The priority of the Archives of Rheumatology is to publish high-quality original research articles, especially in inflammatory rheumatic disorders. In addition to research articles, brief reports, reviews, editorials, letters to the editor can also be published. It is an independent peer-reviewed international journal printed in English. Manuscripts are refereed by a "double-blind peer-reviewed" process for both referees and authors. Editorial Board of the Archives of Rheumatology works under the principles of The World Association of Medical Editors (WAME), the International Council of Medical Journal Editors (ICMJE), and Committee on Publication Ethics (COPE).