Evaluation of antimicrobial efficacy of nanocurcumin-coated gutta-percha against Enterococcus faecalis: An in vitro study.

Q2 Dentistry

Journal of Conservative Dentistry Pub Date : 2023-03-01 Epub Date: 2023-03-16 DOI:10.4103/jcd.jcd_512_22

Deepika Panwar, Kitty Sidhu, Jagat Bhushan, Vandita Kakkar, Manjula Mehta, Jyoti Sharma

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引用次数: 1

Abstract

Background: Nanocurcumin has antimicrobial properties and it is to be tested as a coating on gutta-percha against Enterococcusi faecalis.

Aim: To evaluate the antimicrobial efficacy of nanocurcumin-coated gutta-percha against E. faecalis in comparison with conventional gutta-percha.

Materials and methods: The broth dilution method and colony-forming unit count assay were chosen for the evaluation of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration of nanocurcumin against E. faecalis. ISO size 30, 4% taper gutta-percha cones were manually coated with nanocurcumin. All the coated and noncoated gutta-percha cones were examined under a scanning electron microscope to study the exterior surface. Antibacterial efficacy of nanocurcumin-coated gutta-percha and conventional gutta-percha was seen by agar diffusion method against E. faecalis.

Results: MIC of nanocurcumin was observed at 50 mg/ml for E. faecalis. Nanocurcumin-coated gutta-percha showed a larger zone of inhibition when compared to conventional gutta-percha which showed a smaller zone of inhibition (P < 0.0001). Nanocurcumin-coated gutta-percha showed moderate antimicrobial activity, while conventional gutta-percha showed weak activity.

Conclusion: The results of the study reveal that nanocurcumin has an antimicrobial activity against E. faecalis. The use of herbal alternatives in endodontics might prove to be advantageous.

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纳米姜黄素包被牙胶对粪肠球菌抗菌效果的评价：一项体外研究。

背景：纳米姜黄素具有抗菌性能，并将作为一种涂层在牙胶上进行测试，以对抗粪肠球菌。目的：与传统牙胶相比，评价纳米姜黄素包被牙胶对粪肠球菌的抗菌效果。材料和方法：采用肉汤稀释法和菌落形成单位计数法评价纳米姜黄素对粪肠球菌的最低抑菌浓度（MIC）和最低杀菌浓度。用纳米姜黄素手动涂覆ISO尺寸为30%、4%锥形牙胶锥。在扫描电子显微镜下对所有涂层和未涂层的牙胶锥进行检查，以研究其外表面。用琼脂扩散法观察了纳米姜黄素包被胶胶和传统胶对粪肠球菌的抗菌效果。结果：纳米姜黄素对粪肠球菌的MIC为50mg/ml。与显示较小抑制区的传统牙胶相比，纳米姜黄素涂层的牙胶显示较大的抑制区（P＜0.0001）。纳米姜黄素涂层牙胶显示中等抗菌活性，而传统牙胶显示较弱活性。结论：纳米姜黄素对粪肠球菌具有一定的抗菌活性。在牙髓病治疗中使用草药替代品可能被证明是有利的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Conservative Dentistry Dentistry-Dentistry (all)

CiteScore

2.10

自引率

0.00%

发文量

114

期刊介绍： The Journal of Conservative Dentistry (ISSN - 0972-0707) is the official journal of the Indian Association of Conservative Dentistry and Endodontics (IACDE). Our journal publishes scientific articles, case reports, short communications, invited reviews and comparative studies evaluating materials and methods in the fields of Conservative Dentistry, Dental Materials and Endodontics. J Conserv Dent has a diverse readership that includes full-time clinicians, full-time academicians, residents, students and scientists. Effective communication with this diverse readership requires careful attention to writing style.