Effect of Curcumin on the Head and Neck Squamous Cell Carcinoma Cell Line HN5.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current molecular pharmacology Pub Date : 2023-01-01 DOI:10.2174/1874467215666220414143441

Elaheh Dalir Abdolahinia, Shahin Ahmadian, Sepideh Bohlouli, Faezeh Jafarmadar Gharehbagh, Negar Ghorbani Jahandizi, Sepideh Zununi Vahed, Yalda Rahbar Saadat, Amirala Aghbali, Simin Sharifi, Solmaz Maleki Dizaj, Khalaf F Alsharif, Haroon Khan

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引用次数: 3

Abstract

Background: Curcumin has been isolated from the rhizomes of Curcuma longa. Over the years, it has shown outstanding therapeutic potential in various human disorders, including cancers.

Objective: The aim is to study curcumin's effects on the apoptosis signaling pathway in the head and neck squamous cell carcinoma (HNSCC) cell line HN5.

Methods: The cytotoxicity of curcumin on HN5 cells were assessed. In addition, HN5 cells were also treated with curcumin to evaluate its effect on the caspase-8, -9, Bcl-2, Bax, and Stat3 gene expressions.

Results: The results exhibited that cell viability reduced following curcumin treatment in a concentration- dependent manner. Curcumin treatment caused decreased expression of Bcl2, with simultaneous upregulation of the Bax/Bcl2 ratio. Curcumin increased caspase-9 expression, did not affect caspase-8, and decreased Stat3 expression. The induction of the mitochondria-dependent apoptosis pathway of curcumin happened by modulating the expression of Bcl2 and Bax genes, resulting in the caspase-9 activation. Furthermore, curcumin decreased the expression of the Stat3 in HN-5 cells.

Conclusions: In conclusion, curcumin showed marked anticancer effects in the HN-5 cell line by modulating Stat-3; Bax/Bcl-2 expression in vitro.

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姜黄素对头颈部鳞状细胞癌HN5细胞的影响。

背景:姜黄素是从姜黄根茎中分离得到的。多年来，它在包括癌症在内的各种人类疾病中显示出突出的治疗潜力。目的:研究姜黄素对头颈部鳞状细胞癌HN5细胞凋亡信号通路的影响。方法:观察姜黄素对HN5细胞的细胞毒性。此外，用姜黄素处理HN5细胞，评估其对caspase-8、-9、Bcl-2、Bax和Stat3基因表达的影响。结果:姜黄素处理后，细胞活力呈浓度依赖性降低。姜黄素处理导致Bcl2表达降低，同时Bax/Bcl2比值上调。姜黄素增加caspase-9的表达，不影响caspase-8的表达，降低Stat3的表达。姜黄素通过调节Bcl2和Bax基因的表达，激活caspase-9，从而诱导线粒体依赖的凋亡通路。姜黄素可降低HN-5细胞中Stat3的表达。结论:姜黄素通过调节Stat-3对HN-5细胞株具有明显的抗癌作用;Bax/Bcl-2的体外表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

4.90

自引率

3.70%

发文量

112

期刊介绍： Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.