RET rearrangements are relevant to histopathologic subtypes and clinicopathological features in Thai papillary thyroid carcinoma patients.

IF 2.3 4区 医学 Q3 ONCOLOGY
Thitima Khonrak, Sasithorn Watcharadetwittaya, Yaovalux Chamgramol, Piyapharom Intarawichian, Raksawan Deenonpoe
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引用次数: 0

Abstract

Background: Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The RET gene rearrangements CCDC6::RET and NCOA4::RET are the most common RET gene rearrangements in PTC patients. Different RET::PTC rearrangements are associated with different PTC phenotypes. Methods: Eighty-three formalin-fixed paraffin-embedded (FFPE) PTC samples were examined. The prevalence and expression levels of CCDC6::RET and NCOA4::RET were determined using semi-quantitative polymerase chain reaction (qRT-PCR). The association of these rearrangements with clinicopathological data was investigated. Results: The presence of CCDC6::RET rearrangement was significantly associated with the classic subtype and absence of angio/lymphatic invasion (p < 0.05). While NCOA4::RET was associated with the tall-cell subtype, and presence of angio/lymphatic invasion and lymph node metastasis (p < 0.05). Multivariate analysis demonstrated that an absence of extrathyroidal extension and extranodal extension were independent predictive factors for CCDC6::RET, whereas the tall-cell subtype, large tumor size, angioinvasion, lymphatic invasion and perineural invasion were independent predictive factors for NCOA4::RET (p < 0.05). However, the mRNA expression level of CCDC6::RET and of NCOA4::RET were not significantly associated with clinicopathological data. Conclusion: CCDC6::RET was correlated with an innocent PTC subtype and characteristics, but NCOA4::RET correlated with an aggressive phenotype of PTC. Therefore, these RET rearrangements strongly associated with clinicopathological phenotypes and can be used as predictive markers in PTC patients.

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RET重排与泰国甲状腺乳头状癌患者的组织病理学亚型和临床病理特征有关。
背景:甲状腺乳头状癌(PTC)是最常见的甲状腺癌。RET基因重排CCDC6::RET和NCOA4::RET是PTC患者中最常见的RET基因重排。不同的RET::PTC重排与不同的PTC表型相关。方法:对83份福尔马林固定石蜡包埋(FFPE) PTC样品进行检测。采用半定量聚合酶链反应(qRT-PCR)检测CCDC6::RET和NCOA4::RET的患病率和表达水平。研究了这些重排与临床病理数据的关系。结果:CCDC6::RET重排与经典亚型、无血管/淋巴浸润相关(p < 0.05)。而NCOA4::RET与高细胞亚型、血管/淋巴浸润和淋巴结转移相关(p < 0.05)。多因素分析显示,甲状腺外扩张和结外扩张缺失是CCDC6::RET的独立预测因素,而高细胞亚型、肿瘤大、血管浸润、淋巴浸润和神经周围浸润是NCOA4::RET的独立预测因素(p < 0.05)。然而,CCDC6::RET和NCOA4::RET的mRNA表达水平与临床病理数据无显著相关性。结论:CCDC6::RET与单纯PTC亚型及特征相关,而NCOA4::RET与侵袭性PTC表型相关。因此,这些RET重排与临床病理表型密切相关,可作为PTC患者的预测标志物。
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来源期刊
CiteScore
6.30
自引率
0.00%
发文量
134
审稿时长
4-8 weeks
期刊介绍: Pathology & Oncology Research (POR) is an interdisciplinary Journal at the interface of pathology and oncology including the preclinical and translational research, diagnostics and therapy. Furthermore, POR is an international forum for the rapid communication of reviews, original research, critical and topical reports with excellence and novelty. Published quarterly, POR is dedicated to keeping scientists informed of developments on the selected biomedical fields bridging the gap between basic research and clinical medicine. It is a special aim for POR to promote pathological and oncological publishing activity of colleagues in the Central and East European region. The journal will be of interest to pathologists, and a broad range of experimental and clinical oncologists, and related experts. POR is supported by an acknowledged international advisory board and the Arányi Fundation for modern pathology.
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