MicroRNAs in exhaled breath condensate: A pilot study of biomarker detection for lung cancer

Q3 Medicine

Cancer treatment and research communications Pub Date : 2023-01-01 DOI:10.1016/j.ctarc.2023.100689

Divyanjali Rai , Bijay Pattnaik , Sunil Bangaru , Naveen K. Bhatraju , Jaya Tak , Seetu Kashyap , Umashankar Verma , Rohit Vadala , Geetika Yadav , R S Dhaliwal , Anurag Agrawal , Randeep Guleria , Anant Mohan

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引用次数: 0

Abstract

Introduction

Quantitation of microRNAs secreted by lung cells can provide valuable information regarding lung health. Exhaled breath condensate (EBC) offers a non-invasive way to sample the secreted microRNAs, and could be used as diagnostic tools for lung cancer.

Materials & Methods

EBC samples from twenty treatment-naïve patients with pathologically confirmed lung cancer and twenty healthy subjects were profiled for miRNAs expression. Selected microRNAs were further validated, using quantitative-PCR, in an independent set of 10 subjects from both groups.

Results

A total of 78 miRNAs were found to be significantly upregulated in the EBC of lung cancer patients compared to the control group. Six of these 78 miRNAs were shortlisted for validation. Of these, miR-31–3p, let7i, and miR-449c were significantly upregulated, exhibited good discriminatory power.

Discussion

Differential expression of miRNAs secreted by lung cells could be quantitated in EBC samples, and could be used as a potential non-invasive tool for early diagnosis of lung cancer.

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呼气冷凝物中的microrna:肺癌生物标志物检测的初步研究

引言肺细胞分泌的微小RNA的定量可以提供有关肺部健康的有价值的信息。呼气凝析液（EBC）提供了一种对分泌的微小RNA进行采样的非侵入性方法，可作为癌症的诊断工具。材料&；方法对20例经病理证实的癌症治疗患者和20例健康受试者的EBC样本进行miRNA表达分析。使用定量PCR在两组10名受试者的独立组中进一步验证选定的微小RNA。结果与对照组相比，癌症患者的EBC中共有78个miRNA显著上调。这78个miRNA中有6个入围了验证名单。其中，miR-31-3p、let7i和miR-449c显著上调，表现出良好的辨别力。讨论肺细胞分泌的miRNA的差异表达可以在EBC样品中进行定量，并可作为一种潜在的非侵入性工具用于早期诊断癌症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer treatment and research communications Medicine-Oncology

CiteScore

4.30

自引率

0.00%

发文量

148

审稿时长

56 days

期刊介绍： Cancer Treatment and Research Communications is an international peer-reviewed publication dedicated to providing comprehensive basic, translational, and clinical oncology research. The journal is devoted to articles on detection, diagnosis, prevention, policy, and treatment of cancer and provides a global forum for the nurturing and development of future generations of oncology scientists. Cancer Treatment and Research Communications publishes comprehensive reviews and original studies describing various aspects of basic through clinical research of all tumor types. The journal also accepts clinical studies in oncology, with an emphasis on prospective early phase clinical trials. Specific areas of interest include basic, translational, and clinical research and mechanistic approaches; cancer biology; molecular carcinogenesis; genetics and genomics; stem cell and developmental biology; immunology; molecular and cellular oncology; systems biology; drug sensitivity and resistance; gene and antisense therapy; pathology, markers, and prognostic indicators; chemoprevention strategies; multimodality therapy; cancer policy; and integration of various approaches. Our mission is to be the premier source of relevant information through promoting excellence in research and facilitating the timely translation of that science to health care and clinical practice.