Effects of the Recurrent and Different Doses of Ketamine Exposure on Anxiety-like Behaviors and Locomotor Activity in Juvenile Rats.

IF 1.8 4区医学 Q3 CLINICAL NEUROLOGY

Current Alzheimer research Pub Date : 2023-03-08 DOI:10.2174/1567205020666230308123718

Ayşe Hande Arpacı, Hasan Çalıskan, Emel Güneş, Berrin Işık

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Abstract

Background: Ketamine is a widely used anesthetic agent. Although the potential adverse effects of ketamine use in juvenile age are uncertain, certain studies reported that children exposed to recurrent anesthesia could face an increased risk of neurodevelopmental deficits in motor function and behavioral risks. We aimed to investigate the long-term effects of repeated exposure to various ketamine doses on anxious behavior and locomotor activity in juvenile rats.

Objective: We aimed to investigate the long-term effects of repeated exposure to various ketamine doses on anxious behavior and locomotor activity in juvenile rats.

Methods: Thirty-two Wistar Albino juvenile male rats were randomized into 5 mg/kg, 20 mg/kg, and 50 mg/kg ketamine (KET) and saline (Group C) Groups and KET was administered for 3 consecutive days at 3-hour intervals in 3 doses. Ten days after the last KET dose, behavioral parameters were analyzed with an open field test (OFT), elevated plus maze (EPM), and light-dark box (LDB). Satistical analysis was conducted with Kruskall-Wallis test followed by Dunn's Multiple Comparison Test.

Results: Unsupported rearing behavior decreased in 50 mg/kg KET Groups when compared to Group C. Incorrect transition time, total grooming time, and transfer latency time increased significantly in the 50 mg/kg KET Group when compared to Group C.

Conclusion: These results suggested that 50 mg/kg KET led to anxiety-like behavior and destroyed memory and spatial navigation. Ketamine doses were associated with late effects of ketamine on anxiety- like behavior in juvenile rats. Further studies are needed to determine the mechanisms that play a role in the different effects of ketamine doses on anxiety and memory.

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反复暴露不同剂量氯胺酮对幼鼠焦虑样行为和运动活动的影响

背景：氯胺酮是一种广泛使用的麻醉剂：氯胺酮是一种广泛使用的麻醉剂。尽管氯胺酮对幼鼠的潜在不良影响尚不确定，但有研究报告称，反复暴露于氯胺酮麻醉的儿童可能面临运动功能神经发育缺陷和行为风险增加的风险。我们旨在研究反复暴露于各种酮类药物对幼鼠焦虑行为和运动活动的长期影响：我们旨在研究反复暴露于各种酮类药物对幼年大鼠焦虑行为和运动活动的长期影响：将32只Wistar Albino幼年雄性大鼠随机分为5 mg/kg、20 mg/kg、50 mg/kg氯胺酮（KET）组和生理盐水（C组）组，连续3天给予氯胺酮，每次3小时，共3次给药。最后一次服用氯胺酮 10 天后，用开阔地试验 (OFT)、高架迷宫 (EPM) 和光暗箱 (LDB) 分析行为参数。统计分析采用 Kruskall-Wallis 检验和 Dunn's 多重比较检验：结果：与C组相比，50 mg/kg KET组的无支持饲养行为减少。结果：与 C 组相比，50 毫克/千克 KET 组的无支持饲养行为减少；与 C 组相比，50 毫克/千克 KET 组的不正确过渡时间、总梳理时间和转移潜伏时间显著增加：这些结果表明，50 毫克/千克 KET 会导致焦虑样行为，并破坏记忆和空间导航能力。氯胺酮剂量与氯胺酮对幼鼠焦虑样行为的后期影响有关。要确定氯胺酮剂量对焦虑和记忆产生不同影响的机制，还需要进一步的研究。

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来源期刊

Current Alzheimer research 医学-神经科学

CiteScore

4.00

自引率

4.80%

发文量

审稿时长

4-8 weeks

期刊介绍： Current Alzheimer Research publishes peer-reviewed frontier review, research, drug clinical trial studies and letter articles on all areas of Alzheimer’s disease. This multidisciplinary journal will help in understanding the neurobiology, genetics, pathogenesis, and treatment strategies of Alzheimer’s disease. The journal publishes objective reviews written by experts and leaders actively engaged in research using cellular, molecular, and animal models. The journal also covers original articles on recent research in fast emerging areas of molecular diagnostics, brain imaging, drug development and discovery, and clinical aspects of Alzheimer’s disease. Manuscripts are encouraged that relate to the synergistic mechanism of Alzheimer''s disease with other dementia and neurodegenerative disorders. Book reviews, meeting reports and letters-to-the-editor are also published. The journal is essential reading for researchers, educators and physicians with interest in age-related dementia and Alzheimer’s disease. Current Alzheimer Research provides a comprehensive ''bird''s-eye view'' of the current state of Alzheimer''s research for neuroscientists, clinicians, health science planners, granting, caregivers and families of this devastating disease.