The effect of sinomenine on ERK1/2, JNK and p38 phosphorylation in LPS-stimulated endothelial cells.

IF 0.6 4区医学 Q4 ENDOCRINOLOGY & METABOLISM

Neuro endocrinology letters Pub Date : 2023-03-08

Haifeng Yang, Xiaolan Chen, Yanyan Li, Jing Wang, Feifei Shi, Bin Li, Yiyi Hu

引用次数: 0

Abstract

This study was to investigate the effect of sinomenine by LPS-induced MAPK phosphorylation in endothelial cells. Endothelial cells were challenged with different doses LPS and/or treated with sinomenine at three concentrations (1, 5, or 10 μg/mL) in pathological model, drug safety, treatment and prevention experiments. The cells were incubated at 37 °C in a cell incubator total for 24 h. The lysate cells were collected and analyzed the phosphorylation of ERK1/2, JNK and p38 by MAPK phosphoprotein assay whole cell lysate kit. As expected, LPS could significantly elevated phosphorylation of ERK1/2, JNK and p38, but sinomenine not. The results revealed that sinomenine significantly reduced the phosphorylation of ERK1/2 and p38 in treatment experiment, and inhibited phosphorylation of ERK1/2, JNK and p38 in prevention experiment. Our findings demonstrated that sinomenine protects endothelial cells from LPS-induced inflammation, which might be associated with depressing MAPK signaling pathway.

本刊更多论文

青藤碱对lps刺激内皮细胞ERK1/2、JNK和p38磷酸化的影响。

本研究旨在探讨青藤碱通过lps诱导内皮细胞MAPK磷酸化的作用。采用不同剂量LPS刺激内皮细胞和青藤碱(1、5、10 μg/mL)处理内皮细胞进行病理模型、药物安全性、治疗和预防实验。将细胞在37℃的细胞培养箱中孵育24 h，收集裂解细胞，使用MAPK磷酸化蛋白测定全细胞裂解试剂盒分析ERK1/2、JNK和p38的磷酸化水平。正如预期的那样，LPS可以显著提高ERK1/2、JNK和p38的磷酸化，而青藤碱则没有。结果显示青藤碱在治疗组显著降低ERK1/2和p38的磷酸化水平，在预防组显著抑制ERK1/2、JNK和p38的磷酸化水平。我们的研究结果表明青藤碱可以保护内皮细胞免受lps诱导的炎症，这可能与抑制MAPK信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuro endocrinology letters 医学-内分泌学与代谢

CiteScore

1.00

自引率

14.30%

发文量

审稿时长

6 months

期刊介绍： Neuroendocrinology Letters is an international, peer-reviewed interdisciplinary journal covering the fields of Neuroendocrinology, Neuroscience, Neurophysiology, Neuropsychopharmacology, Psychoneuroimmunology, Reproductive Medicine, Chronobiology, Human Ethology and related fields for RAPID publication of Original Papers, Review Articles, State-of-the-art, Clinical Reports and other contributions from all the fields covered by Neuroendocrinology Letters. Papers from both basic research (methodology, molecular and cellular biology, anatomy, histology, biology, embryology, teratology, normal and pathological physiology, biophysics, pharmacology, pathology and experimental pathology, biochemistry, neurochemistry, enzymology, chronobiology, receptor studies, endocrinology, immunology and neuroimmunology, animal physiology, animal breeding and ethology, human ethology, psychology and others) and from clinical research (neurology, psychiatry and child psychiatry, obstetrics and gynecology, pediatrics, endocrinology, immunology, cardiovascular studies, internal medicine, oncology and others) will be considered. The Journal publishes Original papers and Review Articles. Brief reports, Special Communications, proved they are based on adequate experimental evidence, Clinical Studies, Case Reports, Commentaries, Discussions, Letters to the Editor (correspondence column), Book Reviews, Congress Reports and other categories of articles (philosophy, art, social issues, medical and health policies, biomedical history, etc.) will be taken under consideration.