Identification and expression analysis of C2H2-zinc finger protein genes reveals their role in stress tolerance in Brassica napus.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2023-05-01 DOI:10.1139/gen-2022-0100
Kawalpreet Kaur, Swati Megha, Zhengping Wang, Nat N V Kav, Habibur Rahman
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引用次数: 2

Abstract

C2H2-zinc finger (C2H2-ZF) genes are involved in various biological processes in plants including stress response; however, they lack characterization in Brassica napus. We identified 267 C2H2-ZF genes in B. napus and deciphered their physiological properties, subcellular localization, structure, synteny, and phylogeny and investigated the expression of 20 genes in response to different stresses and phytohormone treatments. The 267 genes were distributed on 19 chromosomes; phylogenetic analysis categorized them into five clades. They varied from 0.41 to 9.2 kb in length, had stress-responsive cis-acting elements in promoter regions, and their protein length varied from 9 to 1366 amino acids. About 42% of the genes had one exon, and 88% genes had orthologs in Arabidopsis thaliana. About 97% of the genes were located in nucleus and 3% in cytoplasmic organelles. qRT-PCR analysis showed a different expression pattern of these genes in response to biotic stresses (Plasmodiophora brassicae and Sclerotinia sclerotiorum) and abiotic stresses (cold, drought, and salinity) and hormonal treatments. Differential expression of the same gene was observed under multiple stress conditions, and a few genes showed similar expression in response to more than one phytohormones. Our results suggest that the C2H2-ZF genes can be targeted for the improvement of stress tolerance in canola.

c2h2 -锌指蛋白基因的鉴定与表达分析揭示了c2h2 -锌指蛋白基因在甘蓝型油菜逆境抗性中的作用。
c2h2 -锌指(C2H2-ZF)基因参与植物的多种生物过程,包括胁迫反应;然而,它们在甘蓝型油菜中缺乏表征。在甘蓝型油菜中鉴定出267个C2H2-ZF基因,并对其生理特性、亚细胞定位、结构、合成和系统发育进行了分析,同时研究了其中20个基因在不同胁迫和植物激素处理下的表达。267个基因分布在19条染色体上;系统发育分析将它们分为五个支系。它们的长度从0.41到9.2 kb不等,在启动子区域具有应激响应的顺式作用元件,蛋白质长度从9到1366个氨基酸不等。在拟南芥中,约42%的基因具有一个外显子,88%的基因具有同源基因。约97%的基因位于细胞核,3%位于细胞器。qRT-PCR分析显示,这些基因在生物胁迫(芸苔菌和菌核菌)、非生物胁迫(寒冷、干旱和盐度)和激素处理下的表达模式不同。在多种胁迫条件下,同一基因的表达存在差异,少数基因对一种以上植物激素的表达相似。结果表明,C2H2-ZF基因可作为油菜抗逆性提高的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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