E3 Ubiquitin Ligase Constitutive Photomorphogenic 1 Regulates Differentiation and Inflammation via MAPK Signaling Pathway in Rabbit Articular Chondrocytes.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Young Seok Eom, Byung Su Ko, Fahad Hassan Shah, Song Ja Kim
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引用次数: 1

Abstract

Constitutive photomorphogenic 1 (COP1), is an E3 ubiquitin ligase that plays a role in the regulation of various cellular processes including cell growth, differentiation, and survival in mammals. In certain conditions such as overexpression or loss of function, COP1 acts either as an oncogenic protein or as a tumor suppressor by targeting specific proteins for ubiquitination-mediated degradation. However, the precise role of COP1 has not been well studied in primary articular chondrocytes. In this study, we investigated the role of COP1 in chondrocyte differentiation. Western blotting and reverse transcription-polymerase chain reaction analysis demonstrated that COP1 overexpression reduced type II collagen expression, promoted cyclooxygenase 2 (COX-2) expression, and reduced sulfated proteoglycan synthesis, as detected by Alcian blue staining. Upon siRNA treatment, revived type II collagen, sulfated proteoglycan production, and decreased COX-2 expression. Phosphorylation of p38 kinase and ERK-1/-2 signaling pathways was regulated by COP1 upon cDNA and siRNA transfection in chondrocytes. The inhibition of the p38 kinase and ERK-1/-2 signaling pathways with SB203580 and PD98059 ameliorated the expression of type II collagen and COX-2 in transfected chondrocytes, thus suggesting that COP1 regulates differentiation and inflammation in rabbit articular chondrocytes via the p38 kinase and ERK-1/-2 signaling pathway.
E3泛素连接酶组成型光形态形成1通过MAPK信号通路调控兔关节软骨细胞的分化和炎症。
组成型光形态形成1 (COP1)是一种E3泛素连接酶,在哺乳动物细胞生长、分化和存活等多种细胞过程中发挥调控作用。在某些情况下,如过度表达或功能丧失,COP1通过靶向泛素化介导降解的特定蛋白作为致癌蛋白或肿瘤抑制蛋白。然而,COP1在原发性关节软骨细胞中的确切作用尚未得到很好的研究。在这项研究中,我们研究了COP1在软骨细胞分化中的作用。Western blotting和逆转录聚合酶链反应分析表明,Alcian blue染色检测到COP1过表达降低了II型胶原蛋白的表达,促进了环氧合酶2 (COX-2)的表达,减少了硫酸蛋白多糖的合成。siRNA处理后,II型胶原蛋白恢复,硫酸盐蛋白多糖生成,COX-2表达降低。转染cDNA和siRNA后,软骨细胞中p38激酶和ERK-1/ 2信号通路的磷酸化受COP1调控。SB203580和PD98059抑制p38激酶和ERK-1/ 2信号通路,改善了II型胶原和COX-2在转染软骨细胞中的表达,提示COP1通过p38激酶和ERK-1/ 2信号通路调节兔关节软骨细胞的分化和炎症。
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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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