Chromosomal Mapping of the Histone Multigene Family and U2 snRNA in Hypancistrus Species (Siluriformes, Loricariidae) from the Brazilian Amazon.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2023-02-01 DOI:10.1089/zeb.2022.0030

Carlos Eduardo Vasconcelos Dos Santos, Bruno Rafael Ribeiro de Almeida, Flávia Dos Santos Tavares, Luan Felipe da Silva Frade, Adauto Lima Cardoso, André Luiz Alves de Sá, Cleusa Yoshiko Nagamachi, Leandro Melo de Sousa, Julio Cesar Pieczarka, Renata Coelho Rodrigues Noronha

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引用次数: 1

Abstract

Loricariidae (Siluriformes) comprises ∼1026 species of neotropical fish, being considered the most diverse among the Siluriformes. Studies on repetitive DNA sequences have provided important data on the evolution of the genomes of members of this family, especially of the Hypostominae subfamily. In this study, the chromosomal mapping of the histone multigene family and U2 snRNA was performed in two species belonging to the Hypancistrus genus, Hypancistrus sp. "pão" (2n = 52, 22m + 18sm +12st) and Hypancistrus zebra (2n = 52, 16m + 20sm +16st). The presence of dispersed signals of histones H2A, H2B, H3, and H4 in the karyotype of both species, with each sequence displaying a varied level of accumulation and dispersion of these sequences between them was observed; in addition, U2 snDNA probe only showed positive results in H. zebra, which present this multigene in the terminal region of three chromosomal pairs. The obtained results resemble data already analyzed in the literature, in which the action of transposable elements interfere in the organization of these multigene families, in addition to other evolutionary processes that shape the evolution of the genome, such as circular or ectopic recombination. This study also shows that the dispersion of the multigene histone family is quite complex, and from this, these data serve as a point of discussion for the evolutionary processes that occur in the Hypancistrus karyotype.

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巴西亚马逊河流域大腹蛇(Siluriformes, Loricariidae)组蛋白多基因家族和U2 snRNA染色体定位

Loricariidae (Siluriformes)包括约1026种新热带鱼，被认为是Siluriformes中最多样化的。重复DNA序列的研究为该科成员的基因组进化提供了重要的数据，特别是下stominae亚科。本研究对Hypancistrus属(Hypancistrus sp.)的两个物种进行了组蛋白多基因家族和U2 snRNA的染色体定位。“p o”(2n = 52,22m + 18sm +12st)和Hypancistrus zebra (2n = 52,16m + 20sm +16st)。组蛋白H2A、H2B、H3和H4在两种物种核型中均存在分散信号，且各序列在两种物种之间表现出不同程度的积累和分散;此外，U2 snDNA探针仅在斑马斑马中显示阳性结果，该多基因存在于三对染色体的末端区。所获得的结果与文献中已经分析的数据相似，其中转座因子的作用干扰了这些多基因家族的组织，以及其他形成基因组进化的进化过程，例如圆形或异位重组。本研究还表明，多基因组蛋白家族的分散是相当复杂的，从这一点来看，这些数据可以作为Hypancistrus核型中发生的进化过程的讨论点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.