In-vitro and In-vivo Identification, Absorbtion and Metabolism Network Analysis of Filifolium sibiricum Flavonoids Dropping Pill by UHPLC-Q-TOF-MS.

IF 2.1 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current drug metabolism Pub Date : 2023-01-01 DOI:10.2174/1389200224666230202144113

Rui-Ting Ma, Ji-Xin Han, Jun-Chan Qiao, Li-Jun Tong, Li-Xia Chen

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引用次数: 1

Abstract

Background: Filifolium sibiricum flavonoids dropping pill (FSFp), a unique Chinese Filifolii sibirici herba extract preparation, has the potential as an alternative therapy against S. aureus infection (SA) and antiinfection. However, its chemical composition and in vivo metabolism characteristics remain unknown, which limits its clinical application.

Methods: Here, we aimed to understand the in vitro and in vivo material basis of FSFp. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to identify chemicals in FSFp as well as its phase I and phase II reaction metabolites in plasma, urine and feces.

Results: A total of 38 chemicals were characterized in FSFp, including 22 flavonoids, 10 organic acids, 3 chromones, 1 aromatic ketone, 1 coumarin, and 1 ligan. After analysis of the drugged bio-samples, a total of 21 compounds were found in urine, and 16 of them were found in feces, but only one was found in plasma. In addition, 56 FSFp-related metabolites were characterized, of which 56 were in urine, 4 in feces, and 8 in plasma.

Conclusion: This is the first comprehensive research of FSFp on chemical constituents and metabolic profiles. It was expected that this study would offer reliable support for further investigation of FSFp.

查看原文本刊更多论文

UHPLC-Q-TOF-MS法分析西伯利亚黄酮类化合物滴丸的体内体外鉴定、吸收及代谢网络。

背景:西伯利亚黄芪黄酮滴丸(FSFp)是一种独特的中国西伯利亚黄芪提取物制剂，具有抗金黄色葡萄球菌感染和抗感染的潜力。但其化学成分和体内代谢特性尚不清楚，限制了其临床应用。方法:了解FSFp的体内外物质基础。采用超高效液相色谱-四极杆飞行时间质谱联用(UHPLC-Q-TOF-MS)对FSFp中的化学成分及其在血浆、尿液和粪便中的I、II期反应代谢物进行了鉴定。结果:FSFp共鉴定出38种化学成分，包括22种黄酮类化合物、10种有机酸、3种色素、1种芳香酮、1种香豆素和1种木脂素。对服用药物的生物样本进行分析后，在尿液中发现了21种化合物，在粪便中发现了16种化合物，但在血浆中只发现了1种。此外，鉴定出56种fsfp相关代谢物，其中尿中56种，粪便中4种，血浆中8种。结论:本研究首次对FSFp的化学成分和代谢谱进行了全面的研究。期望本研究能为FSFp的进一步研究提供可靠的支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current drug metabolism 医学-生化与分子生物学

CiteScore

4.30

自引率

4.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Current Drug Metabolism aims to cover all the latest and outstanding developments in drug metabolism, pharmacokinetics, and drug disposition. The journal serves as an international forum for the publication of full-length/mini review, research articles and guest edited issues in drug metabolism. Current Drug Metabolism is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the most important developments. The journal covers the following general topic areas: pharmaceutics, pharmacokinetics, toxicology, and most importantly drug metabolism. More specifically, in vitro and in vivo drug metabolism of phase I and phase II enzymes or metabolic pathways; drug-drug interactions and enzyme kinetics; pharmacokinetics, pharmacokinetic-pharmacodynamic modeling, and toxicokinetics; interspecies differences in metabolism or pharmacokinetics, species scaling and extrapolations; drug transporters; target organ toxicity and interindividual variability in drug exposure-response; extrahepatic metabolism; bioactivation, reactive metabolites, and developments for the identification of drug metabolites. Preclinical and clinical reviews describing the drug metabolism and pharmacokinetics of marketed drugs or drug classes.