Comparison of a Minimally Invasive Transthoracic Approach and a Surgical Method For Intrapleural Injection of Tumor Cells in Mice.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2023-04-01 Epub Date: 2023-03-15 DOI:10.30802/AALAS-CM-22-000044
Jiajie Jessica Xu, Melissa Y Lucero, Nicole L Herndon, Michael C Lee, Jefferson Chan
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引用次数: 0

Abstract

Intrapleural injections can be used in mice to deliver therapeutic and diagnostic agents and to model human disease processes (for example, pleural fluid accumulation, malignant pleural disease, and lung cancers). In the context of establishing cancer models, minimally invasive methods of intrapleural injection are desirable because inflammation at the injection site can have a major impact on tumor growth and progression. Common approaches for intrapleural injection include surgical exposure of the thoracic wall or the diaphragm prior to injection; however, these invasive procedures require tissue dissection that triggers an undesirable inflammatory response and increases the risk of pneumothorax. While nonsurgical procedures can minimize this concern, 'blind' injections may lead to off-target inoculation. In this study, we hypothesized that a minimally invasive transthoracic approach (MI-TT) would produce a tumor distribution and burden similar to that of a surgical transabdominal approach (SX-TA). Prior to performing the procedures on live mice, surgeons were trained using cadavers and terminal procedures. Then a total of 14 nude mice (female, 4 to 6 wk old) were injected with 50 μL (5 million) A549-Luc2 human cancer cells either using the MI-TT (n = 8) or SX-TA (n = 6) approach under carprofen analgesia and isoflurane anesthesia. Our results indicate that with training, a minimally invasive transthoracic approach for intrapleural injection provides more consistent tumor placement and a greater tumor burden than does the surgical method. However, additional studies are necessary to confirm anatomic placement and characterize tumor profiles.

微创经胸入路和手术方法在小鼠胸膜内注射肿瘤细胞的比较。
胸膜内注射可用于小鼠递送治疗剂和诊断剂,并模拟人类疾病过程(例如,胸腔积液、恶性胸膜疾病和肺癌)。在建立癌症模型的背景下,胸膜内注射的微创方法是可取的,因为注射部位的炎症可能对肿瘤生长和进展产生重大影响。胸膜内注射的常见方法包括在注射前手术暴露胸壁或膈肌;然而,这些侵入性手术需要进行组织解剖,这会引发不理想的炎症反应,并增加患肺气肿的风险。虽然非手术程序可以最大限度地减少这种担忧,但“盲目”注射可能会导致脱靶接种。在这项研究中,我们假设微创经胸入路(MI-TT)会产生与外科经腹部入路(SX-TA)相似的肿瘤分布和负担。在对活体小鼠进行手术之前,外科医生接受了尸体和末端手术的训练。然后用MI-TT(n=8)或SX-TA(n=6)方法,在卡洛芬镇痛和异氟烷麻醉下,向总共14只裸鼠(雌性,4至6周大)注射50μL(500万)A549-Luc2人癌症细胞。我们的研究结果表明,通过训练,胸膜内注射的微创经胸入路比手术方法提供了更一致的肿瘤放置和更大的肿瘤负担。然而,需要进行更多的研究来确认解剖位置和肿瘤特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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