Oral Inoculation of Point-of-Lay Hens with the New South Wales Outbreak Strain of Salmonella Enteritidis Phage Type 12 Causes Infection, but Minimal Histopathology.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Alison Collins, Anne Jordan, Yuanshuo Gao, Peter Groves
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引用次数: 0

Abstract

An outbreak of food poisoning in New South Wales (NSW) Australia in 2018, caused by Salmonella enterica serovar Enteritidis phage type 12 (PT12), was traced to eggs consumed from a NSW layer flock. This was the first report of Salmonella Enteritidis infection in NSW layer flocks, despite ongoing environmental monitoring. Clinical signs and mortalities were minimal in most flocks, although seroconversion and infection were demonstrated in some flocks. An oral dose-response challenge study with Salmonella Enteritidis PT12 was undertaken in commercial point-of-lay hens. Cloacal swabs collected at 3, 7, 10, and 14 days postinoculation and caeca, liver, spleen, ovary, magnum, and isthmus tissues collected at necropsy at either 7 or 14 days were processed for Salmonella isolation (AS 5013.10-2009 from ISO6579:2002). Histopathology was performed on the above tissues, as well as lung, pancreas, kidney, heart, and additional intestinal and reproductive tract tissues. Salmonella Enteritidis was consistently detected in cloacal swabs between 7 and 14 days postchallenge. The Salmonella Enteritidis PT12 isolate successfully colonized the gastrointestinal tract, liver, and spleen of all hens orally challenged with 107, 108, and 109 Salmonella Enteritidis, and less consistently colonized their reproductive tracts. On histopathology, mild lymphoid hyperplasia in the liver and spleen, along with hepatitis, typhlitis, serositis, and salpingitis, was observed at 7 and 14 days postchallenge, with a greater proportion of affected birds in the two higher dose groups. Diarrhea and culture of Salmonella Enteritidis from heart blood were not detected in challenged layers. The NSW isolate of Salmonella Enteritidis PT12 was able to invade and colonize the birds' reproductive tracts as well as a wide range of other tissues, indicating the potential for these naive commercial hens to contaminate their eggs.

口服接种新南威尔士州12型肠炎沙门氏菌爆发菌株的蛋鸡引起感染,但组织病理学最小。
2018年,澳大利亚新南威尔士州爆发了由肠道沙门氏菌血清型肠炎噬菌体12型(PT12)引起的食物中毒事件,可追溯到新南威尔士州蛋鸡群食用的鸡蛋。尽管正在进行环境监测,但这是新南威尔士州蛋鸡中首次报告肠炎沙门氏菌感染。大多数禽群的临床症状和死亡率都很低,尽管在一些禽群中出现血清转化和感染。在商业点蛋鸡中进行了肠炎沙门氏菌PT12口服剂量反应挑战研究。接种后3、7、10和14天收集的肛肠拭子和7或14天尸检时收集的caeca、肝脏、脾脏、卵巢、magnum和峡部组织进行沙门氏菌分离处理(来自ISO6579:2002的AS 5013.10-2009)。对上述组织,以及肺、胰腺、肾脏、心脏和其他肠道和生殖道组织进行组织病理学检查。肠炎沙门氏菌在攻毒后7至14天内持续在肛肠拭子中检测到。肠炎沙门氏菌PT12分离物在所有经口感染107、108和109肠炎沙门氏菌的母鸡的胃肠道、肝脏和脾脏成功定植,在生殖道的定植不太一致。在组织病理学上,在攻毒后7天和14天观察到肝脏和脾脏轻度淋巴样增生,并伴有肝炎、斑疹伤寒、浆液炎和输卵管炎,两个高剂量组的受感染鸟类比例更高。攻毒蛋鸡未检出腹泻和心脏血肠炎沙门氏菌培养。新南威尔士州分离的肠炎沙门氏菌PT12能够侵入并定殖鸟类的生殖道以及广泛的其他组织,这表明这些幼稚的商业母鸡有可能污染它们的鸡蛋。
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来源期刊
Avian Diseases
Avian Diseases 农林科学-兽医学
CiteScore
2.40
自引率
7.10%
发文量
80
审稿时长
3 months
期刊介绍: Avian Diseases is an international journal dedicated to publishing original basic or clinical research of the highest quality from various disciplines including microbiology, immunology, pathology and epidemiology. Papers on avian diseases relevant to etiology, pathogenesis, diagnosis, treatment, and control are accepted. Manuscripts dealing with avian species other than poultry will be considered only if the subject is relevant to poultry health.
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