A reliable elasticity sensing method for analysis of cell entosis using microfluidic cytometer.

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Biomedical Engineering Letters Pub Date : 2023-02-04 eCollection Date: 2023-05-01 DOI:10.1007/s13534-023-00264-0

Jifeng Ren, Lei Fan

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引用次数: 0

Abstract

Cell entosis is a novel cell death process starting from cell-in-cell invasion. In general, cancer cells own higher incidence rate of cell entosis comparing to non-cancerous cells. Studies arguing whether cell entosis is a tumor suppressing process or a tumor accelerating process can deepen our understanding of tumor development. Cell elasticity is recognized as one of tumor malignant biomarkers. There have been some researchers studying cell elasticity in cell entosis. However, existing cell elasticity sensing technique (i.e. micropipette aspiration) can hardly be reliable neither high-throughput. In this work, we introduce an elasticity sensing method for quantifying both cell elasticity in cell-in-cell structures and single floating cells using a microfluidic cytometer. We not only argue our cell elasticity sensing method is reliable for already occurred entosis but also apply such method on predicting the "outer" cells in entosis of different cell types. The elasticity sensing method proposed in this manuscript is able to provide an effective and reliable way to further study deeper mechanism in cell entosis.

Supplementary information: The online version contains supplementary material available at 10.1007/s13534-023-00264-0.

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利用微流体细胞仪分析细胞内生的可靠弹性传感方法。

细胞内陷是一种新型的细胞死亡过程，始于细胞间的侵袭。一般来说，与非癌细胞相比，癌细胞的细胞内陷发生率更高。研究细胞内陷是抑制肿瘤的过程还是加速肿瘤的过程，可以加深我们对肿瘤发生发展的认识。细胞弹性是公认的肿瘤恶性生物标志物之一。已有一些研究人员对细胞内生过程中的细胞弹性进行了研究。然而，现有的细胞弹性传感技术（即微量吸液管吸液技术）既不可靠，也无法实现高通量。在这项工作中，我们介绍了一种弹性传感方法，可利用微流体细胞仪量化细胞间结构和单个漂浮细胞的细胞弹性。我们不仅论证了我们的细胞弹性传感方法对于已经发生的内生现象是可靠的，而且还将这种方法应用于预测不同类型细胞内生现象中的 "外层 "细胞。本稿件提出的弹性传感方法能够为进一步研究细胞内陷的深层机制提供有效而可靠的途径：在线版本包含补充材料，可在10.1007/s13534-023-00264-0查阅。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Engineering Letters ENGINEERING, BIOMEDICAL-

CiteScore

6.80

自引率

0.00%

发文量

期刊介绍： Biomedical Engineering Letters (BMEL) aims to present the innovative experimental science and technological development in the biomedical field as well as clinical application of new development. The article must contain original biomedical engineering content, defined as development, theoretical analysis, and evaluation/validation of a new technique. BMEL publishes the following types of papers: original articles, review articles, editorials, and letters to the editor. All the papers are reviewed in single-blind fashion.